| Title: |
The Use of a Reference Allergenic Extract in the Evaluation of Allergen Products. |
| Authors: |
Ingemann, L.; Formgren, H.; Løwenstein, H.; Ipsen, H. |
| Source: |
Allergy; May85, Vol. 40 Issue 4, p273-281, 9p |
| Subject Terms: |
Artemisia vulgaris; Artemisia; Allergens; Immunoelectrophoresis; Electrophoresis; Immunodiagnosis |
| Abstract: |
Two commercially available mugwort (Artemisia vulgańs (Av)) pollen extracts Av-ALK and As-PS were compared with a dialysed reference mugwort pollen extract (Av-REF) by means of crossed immunoelectrophoresis (CIE), crossed radio immunoelectrophoresis (CR1E), PAST-inhibition, PAST-screening and skin prick test. Av-ALK and Av-PS both contained approximately half the number of precipitation arcs detectable in Av-REF, and Av-PS seemed to lack two intermediate allergens and two minor allergens, while Av-ALK seemed to lack one minor allergen. The RAST-inhibition curve obtained for Av-PS did not parallel the curves obtained for Av-A Lit and Ay-REF, indicating that the allergen composition of Av-PS differs from that of the others. Further, EAST-screening experiments support the CIE observation that Av-PS seemed to lack two of the intermediate allergens. The HEP values determined for Av-ALK and Av-PS diverge considerably from those stated by the manufacturers. The results obtained demonstrate that the outcome of a comparison between a reference allergenic extract and allergenic products depends very much on the method employed. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |