| Title: |
Comparisons between skeletal muscle imaging techniques and histology in tracking midthigh hypertrophic adaptations following 10 wk of resistance training. |
| Authors: |
Ruple, Bradley A.; Smith, Morgan A.; Osburn, Shelby C.; Sexton, Casey L.; Godwin, Joshua S.; Edison, Joseph L.; Poole, Christopher N.; Stock, Matt S.; Fruge, Andrew D.; Young, Kaelin C.; Roberts, Michael D. |
| Source: |
Journal of Applied Physiology; Aug2022, Vol. 133 Issue 2, p416-425, 10p |
| Subject Terms: |
Resistance training; Histological techniques; Skeletal muscle; Dual-energy X-ray absorptiometry; Lean body mass |
| Abstract: |
This study had two aims. Aim1 was to determine the agreement between midthigh vastus lateralis (VL) cross-sectional area measured by ultrasound (mCSAUS) versus magnetic resonance imaging (mCSAMRI) at a single time point, and the ability of each to detect hypertrophic changes. Aim2 was to assess the relationships between pre- and posttraining changes in thigh lean mass determined by dual-energy X-ray absorptiometry (DXA), VL mCSAUS, ultrasound-determined VL thickness (VLThick), and VL mean myofiber crosssectional area (fCSA) with changes in VL mCSAMRI. Twelve untrained males (age: 20± 1 yr, BMI: 26.9 ± 5.4 kg/m²; n = 12) engaged in a 10-wk resistance training program (2×/week) where right midthigh images and VL biopsies were obtained before and 72 h following the last training bout. Participants' VL mCSAMRI (P = 0.005), DXA thigh lean mass (P = 0.015), and VLThick (P = 0.001) increased following training, whereas VL mCSAUS and fCSA did not. For Aim1, mCSAUS demonstrated excellent concordance [concordance correlation coefficients (CCC) = 0.830] with mCSAMRI, albeit mCSAUS values were systematically lower compared with mCSAMRI (mean bias: -2.29 cm²). In addition, PRE-to-POST VL mCSA changes between techniques exhibited good agreement (CCC = 0.700; mean bias: -1.08 cm²). For Aim², moderate, positive correlations existed for pre-to-post changes in VL mCSAMRI and DXA thigh lean mass (r = 0.580, P = 0.048), mCSAUS (r = 0.622, P = 0.031), and VLThick (r = 0.520, P = 0.080). A moderate, negative correlation existed between mCSAMRI and fCSA (r = -0.569, P = 0.054). Our findings have multiple implications: 1) resistance training-induced hypertrophy was dependent on the quantification method, 2) ultrasound-determined mCSA shows good agreement with MRI, and 3) tissuelevel changes poorly agreed with mean fCSA changes and this requires further research. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |