Calcium-responsive contractility during fertilization in sea urchin eggs.
| Title: | Calcium-responsive contractility during fertilization in sea urchin eggs. |
|---|---|
| Authors: | Stack C; Department of Biology, New Mexico State University, Las Cruces, NM 88003-8001, USA.; Lucero AJ; Shuster CB |
| Source: | Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2006 Apr; Vol. 235 (4), pp. 1042-52. |
| Publication Type: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Wiley Country of Publication: United States NLM ID: 9201927 Publication Model: Print Cited Medium: Print ISSN: 1058-8388 (Print) Linking ISSN: 10588388 NLM ISO Abbreviation: Dev Dyn Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New York, NY : Wiley, c1992- |
| MeSH Terms: | Fertilization*; Calcium/*metabolism ; Cytoskeleton/*metabolism ; Oocytes/*cytology; Actins/metabolism ; Myosin Type II/metabolism ; Animals ; Fluorescent Dyes ; Microinjections ; Microscopy, Confocal ; Microscopy, Video ; Phalloidine ; Rhodamines ; Sea Urchins |
| Abstract: | Fertilization triggers a reorganization of oocyte cytoskeleton, and in sea urchins, there is a dramatic increase in cortical F-actin. However, the role that myosin II plays during fertilization remains largely unexplored. Myosin II is localized to the cortical cytoskeleton both before and after fertilization and to examine myosin II contractility in living cells, Lytechinus pictus eggs were observed by time-lapse microscopy. Upon sperm binding, a cell surface deflection traversed the egg that was followed by and dependent on the calcium wave. The calcium-dependence of surface contractility could be reproduced in unfertilized eggs, where mobilization of intracellular calcium in unfertilized eggs under compression resulted in a marked contractile response. Lastly, inhibition of myosin II delayed absorption of the fertilization cone, suggesting that myosin II not only responds to the same signals that activate eggs but also participates in the remodeling of the cortical actomyosin cytoskeleton during the first zygotic cell cycle.; ((c) 2006 Wiley-Liss, Inc.) |
| References: | Curr Biol. 2005 Apr 26;15(8):724-31. (PMID: 15854904); J Cell Biol. 1980 Dec;87(3 Pt 1):771-82. (PMID: 6893988); Gene. 1997 Oct 1;198(1-2):359-65. (PMID: 9370302); Eur J Cell Biol. 2000 Feb;79(2):81-91. (PMID: 10727016); Development. 1999 Jun;126(14):3101-17. (PMID: 10375502); Dev Biol. 1998 Jan 15;193(2):195-208. (PMID: 9473324); J Cell Biol. 1979 Oct;83(1):241-8. (PMID: 41844); J Cell Biol. 1997 Oct 20;139(2):397-415. (PMID: 9334344); J Biol Chem. 1999 Jul 16;274(29):20643-9. (PMID: 10400696); Mech Dev. 2004 Mar;121(3):225-35. (PMID: 15003626); Curr Biol. 2005 Apr 26;15(8):732-6. (PMID: 15854905); Nature. 2000 Aug 10;406(6796):633-6. (PMID: 10949304); J Cell Biol. 1990 May;110(5):1589-98. (PMID: 2335565); Zygote. 1993 Feb;1(1):35-42. (PMID: 8081800); Science. 1987 May 29;236(4805):1081-6. (PMID: 3576221); Dev Growth Differ. 1980;22(3):517-530. (PMID: 37282020); J Cell Biol. 2000 Feb 21;148(4):755-67. (PMID: 10684256); Science. 2003 Mar 14;299(5613):1743-7. (PMID: 12637748); J Cell Biol. 1980 Sep;86(3):803-9. (PMID: 6893332); J Cell Sci. 2004 Dec 1;117(Pt 25):5995-6005. (PMID: 15536121); Biol Reprod. 1998 Mar;58(3):867-73. (PMID: 9510978); Curr Top Dev Biol. 1992;26:9-21. (PMID: 1563280); Cell Motil Cytoskeleton. 1997;36(1):30-42. (PMID: 8986375); J Cell Biol. 1978 May;77(2):536-50. (PMID: 25901); J Biol Chem. 1986 Apr 25;261(12):5389-95. (PMID: 2937787); Cell Motil Cytoskeleton. 2003 Dec;56(4):252-66. (PMID: 14584028); Zygote. 1995 Aug;3(3):251-8. (PMID: 8903794); Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10613-7. (PMID: 1438256); J Cell Biol. 1987 Aug;105(2):927-36. (PMID: 3624311); Cell Motil Cytoskeleton. 1988;9(1):85-96. (PMID: 3356047); Cell Struct Funct. 1996 Dec;21(6):475-82. (PMID: 9078405); Ann N Y Acad Sci. 1990;582:22-30. (PMID: 2192596); Dev Biol. 1993 May;157(1):157-69. (PMID: 8482408); J Cell Sci. 1997 Jun;110 ( Pt 12):1373-86. (PMID: 9217323); Dev Biol. 1985 Sep;111(1):260-5. (PMID: 2411615); J Biol Chem. 2003 Apr 4;278(14):12247-54. (PMID: 12540836); Dev Biol. 1981 May;84(1):1-26. (PMID: 6265303); J Cell Biol. 1999 Sep 6;146(5):981-92. (PMID: 10477753); Dev Biol. 2002 Jan 1;241(1):1-23. (PMID: 11784091); Dev Biol. 1980 Aug;78(2):435-49. (PMID: 6893311); Mol Biol Cell. 1998 Sep;9(9):2509-25. (PMID: 9725909); J Cell Biol. 1988 Dec;107(6 Pt 1):2181-9. (PMID: 2461948); Development. 1995 Oct;121(10):3457-66. (PMID: 7588078); Curr Opin Cell Biol. 1999 Feb;11(1):26-33. (PMID: 10047526); Dev Biol. 1999 Jul 15;211(2):157-76. (PMID: 10395780); Cell Motil Cytoskeleton. 1987;7(1):46-53. (PMID: 3815543); J Biochem. 1996 May;119(5):906-13. (PMID: 8797090); Nature. 1976 May 6;261(5555):68-71. (PMID: 944858); Dev Biol. 2004 May 15;269(2):421-32. (PMID: 15110710); J Muscle Res Cell Motil. 2004;25(4-5):337-41. (PMID: 15548862); Mol Biol Cell. 1999 Dec;10(12):4075-90. (PMID: 10588644); Cell Motil Cytoskeleton. 2001 Apr;48(4):245-61. (PMID: 11276074); Exp Cell Res. 1979 Aug;122(1):203-18. (PMID: 582584); Dev Biol. 1983 Jan;95(1):244-8. (PMID: 6825929); J Cell Biol. 1977 Jul;74(1):251-63. (PMID: 141455); Dev Biol. 1979 Jun;70(2):306-26. (PMID: 478164); J Cell Sci. 1994 Aug;107 ( Pt 8):2239-48. (PMID: 7983183); J Cell Biol. 1984 Nov;99(5):1647-54. (PMID: 6490715); Gamete Res. 1986;14:277-91. (PMID: 11540931); Cell Motil Cytoskeleton. 1996;34(1):48-56. (PMID: 8860231); Dev Biol. 1989 Aug;134(2):327-41. (PMID: 2663573); J Cell Biol. 1983 Dec;97(6):1745-52. (PMID: 6227627); Methods Cell Biol. 1999;61:439-72. (PMID: 9891327); Semin Cell Dev Biol. 2001 Feb;12(1):45-51. (PMID: 11162746); J Cell Biol. 1993 Nov;123(3):637-52. (PMID: 8227130); Curr Top Dev Biol. 1995;31:101-37. (PMID: 8746663); Dev Biol. 1982 Jan;89(1):128-37. (PMID: 7198594); Dev Biol. 2002 May 15;245(2):237-54. (PMID: 11977978); J Cell Biol. 1982 Jul;94(1):165-78. (PMID: 6181080); Dev Biol. 1988 Jun;127(2):338-48. (PMID: 3378667); Dev Growth Differ. 1990 Oct;32(5):549-556. (PMID: 37281870); Science. 1987 May 29;236(4805):1086-91. (PMID: 3576222); Biorheology. 1970 Jan;6(3):201-34. (PMID: 4907513); Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1472-7. (PMID: 15671182) |
| Grant Information: | GM 61222 United States GM NIGMS NIH HHS; U54 CA132383 United States CA NCI NIH HHS; S06 GM008136 United States GM NIGMS NIH HHS; S06 GM 08136 United States GM NIGMS NIH HHS; R25 GM061222 United States GM NIGMS NIH HHS; U56 CA096286 United States CA NCI NIH HHS |
| Substance Nomenclature: | 0 (Actins); 0 (Fluorescent Dyes); 0 (Rhodamines); 17466-45-4 (Phalloidine); EC 3.6.1.- (Myosin Type II); SY7Q814VUP (Calcium) |
| Entry Date(s): | Date Created: 20060214 Date Completed: 20060626 Latest Revision: 20250529 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC2566787 |
| DOI: | 10.1002/dvdy.20695 |
| PMID: | 16470603 |
| Database: | MEDLINE |
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't