PTCA determination in human hair: reliability and analytical aspects.
| Title: | PTCA determination in human hair: reliability and analytical aspects. |
|---|---|
| Authors: | Zanetti R; CPO-Piedmont Cancer Registry, Turin, Italy. roberto.zanetti@cpo.it; Zoccola M; Mossotti R; Innocenti R; Loria DI; Rosso S |
| Corporate Authors: | Helios 2 Working Group |
| Source: | Medical science monitor : international medical journal of experimental and clinical research [Med Sci Monit] 2006 Jun; Vol. 12 (6), pp. PI23-8. Date of Electronic Publication: 2006 May 29. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't; Validation Study |
| Language: | English |
| Journal Info: | Publisher: International Scientific Information, Inc Country of Publication: United States NLM ID: 9609063 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1234-1010 (Print) Linking ISSN: 12341010 NLM ISO Abbreviation: Med Sci Monit Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2014- : Smithtown, NY : International Scientific Information, Inc.; Original Publication: Warsaw, Poland : Medical Science International |
| MeSH Terms: | Chromatography, High Pressure Liquid/*standards ; Hair/*chemistry ; Melanoma/*diagnosis ; Pyrroles/*analysis; Female ; Humans ; Male ; Reproducibility of Results ; Risk |
| Abstract: | Background: In this study we analysed the reliability of HPLC determination of 2.3.5-pyrroletricarboxylic acid (PTCA). This product derives from oxidation of melanin in human hair, and is a good candidate as a risk marker for skin tumors.; Material/methods: We determined PTCA in 100 melanoma cases and 100 controls, 21 replicates from six different reference hairs, two trace elements, and one reference sample (brown hair).; Results: Work-up procedures showed an almost perfect reproducibility with an Intraclass Correlation Coefficient (ICC) of 0.990. We noticed a low, detectable, but not statistically significant decrease in reproducibility proportional to the amount of PTCA. Agreement between determination following injection of the same solution in HPLC column was also high, with an overall ICC of 0.986. Simultaneous analysis of reproducibility showed a partial ICC for work-up (0.986), for injection (0.987), and an overall standardised ICC (0.975). The analysis of the two reference tracers in successive tests showed a weak, not statistically significant, decreasing linear drift, possibly due to various factors, such as aging of chemical solutions and HPLC columns.; Conclusions: PTCA extracted from human hair through oxidation and determined with HPLC can be considered a reliable marker as a candidate for identifying persons at high risk for melanoma. |
| Substance Nomenclature: | 0 (Pyrroles); 945-32-4 (pyrrole-2,3,5-tricarboxylic acid) |
| Entry Date(s): | Date Created: 20060531 Date Completed: 20060811 Latest Revision: 20191210 |
| Update Code: | 20260130 |
| PMID: | 16733495 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't; Validation Study