[Assembly of viral particles in the cells infected with endotheliotropic or fibroblastotropic strains of human cytomegalovirus].
| Title: | [Assembly of viral particles in the cells infected with endotheliotropic or fibroblastotropic strains of human cytomegalovirus]. |
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| Authors: | Riaskina SS; Boiarkin AV; Bystrevskaia VB |
| Source: | Voprosy virusologii [Vopr Virusol] 2006 Mar-Apr; Vol. 51 (2), pp. 39-44. |
| Publication Type: | English Abstract; Journal Article |
| Language: | Russian |
| Journal Info: | Publisher: FBUN T︠S︡NII Ėpidemiologii Rospotrebnadzora Country of Publication: Russia (Federation) NLM ID: 0417337 Publication Model: Print Cited Medium: Print ISSN: 0507-4088 (Print) Linking ISSN: 05074088 NLM ISO Abbreviation: Vopr Virusol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Moskva : FBUN T︠S︡NII Ėpidemiologii Rospotrebnadzora; Original Publication: Moskva : Ministerstvo zdravookhranenii︠a︡ SSSR |
| MeSH Terms: | Virus Assembly*; Cytomegalovirus/*physiology ; Cytomegalovirus Infections/*virology ; Endothelium, Vascular/*virology ; Fibroblasts/*virology; Capsid/physiology ; Capsid/ultrastructure ; Cell Nucleus/virology ; Cytomegalovirus/ultrastructure ; Cytoplasm/virology ; DNA, Viral/analysis ; Cells, Cultured ; Humans ; In Situ Hybridization ; Lung ; Microscopy, Electron ; Umbilical Veins |
| Abstract: | The ultrastructure of endothelial cells and fibroblasts infected with VHL/E or AD169 strains was examined. The cells producing the virus 25 days after infection with VHL/E strains (0.01 PFU/cell) contain numerous cytoplasmic-located filament-associated capsids in addition to standard infectious and noninfectious viral particles. Only single filament-contacting capsids were found in the cytoplasm of the cells producing the virus 20 days after infection with AD169 strain (0.001 PFU/cell). In situ DNA hydridization on the VHL/E-infected cells has indicated that the capsid-contacting filament is human cytomegalovirus DNA. The findings suggest that premature release of viral DNA from the capsids occurs during their maturation in the cytoplasm. |
| Substance Nomenclature: | 0 (DNA, Viral) |
| Entry Date(s): | Date Created: 20060608 Date Completed: 20060717 Latest Revision: 20161109 |
| Update Code: | 20260130 |
| PMID: | 16756178 |
| Database: | MEDLINE |
English Abstract; Journal Article