Rapid three-dimensional imaging of individual insulin release events by Nipkow disc confocal microscopy.
| Title: | Rapid three-dimensional imaging of individual insulin release events by Nipkow disc confocal microscopy. |
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| Authors: | Rutter GA; Henry Wellcome Signalling Laboratories and Department of Biochemistry, School of Medical Sciences, University Walk, University of Bristol, Bristol, BS8 1TD, UK. g.a.rutter@bris.ac.uk; Loder MK; Ravier MA |
| Source: | Biochemical Society transactions [Biochem Soc Trans] 2006 Nov; Vol. 34 (Pt 5), pp. 675-8. |
| Publication Type: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Portland Press On The Behalf Of The Biochemical Society Country of Publication: England NLM ID: 7506897 Publication Model: Print Cited Medium: Print ISSN: 0300-5127 (Print) Linking ISSN: 03005127 NLM ISO Abbreviation: Biochem Soc Trans Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Portland Press On The Behalf Of The Biochemical Society |
| MeSH Terms: | Exocytosis/*physiology ; Insulin/*metabolism ; Microscopy, Confocal/*methods; Cell Membrane/metabolism ; Insulin-Secreting Cells/metabolism ; Animals ; Cell Line ; Image Processing, Computer-Assisted ; Insulin Secretion |
| Abstract: | Minute-to-minute control of the release of insulin by pancreatic beta-cells in response to glucose or other stimuli requires the precise delivery of large dense-core vesicles to the plasma membrane and regulated exocytosis. At present, the precise spatial organization at the cell surface and the nature of these events ('transient' versus 'full fusion') are debated. In order to monitor secretory events simultaneously over most of the surface of clusters of single MIN6 beta-cells, we have expressed recombinant neuropeptide Y-Venus (an enhanced and vesicle-targeted form of yellow fluorescent protein) as an insulin surrogate. Individual exocytotic events were monitored using Nipkow spinning disc confocal microscopy, with acquisition of a three-dimensional complete image (eight to twelve confocal slices) in |
| Grant Information: | United Kingdom Wellcome Trust |
| Substance Nomenclature: | 0 (Insulin) |
| Entry Date(s): | Date Created: 20061021 Date Completed: 20070129 Latest Revision: 20181217 |
| Update Code: | 20260130 |
| DOI: | 10.1042/BST0340675 |
| PMID: | 17052172 |
| Database: | MEDLINE |
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't