| Title: |
Effect of Mito-TEMPO on Post-thawed Semen Quality in Goats. |
| Alternate Title: |
اثر میتوتمپو بر کیفیت منی بز پس از ی خگشایی. (Persian) |
| Authors: |
Barfourooshi, Hoda Javaheri; Esmaeilkhanian, Saeid; Davachi, Navid Dadashpour; Asadzadeh, Nader; Masoudi, Reza |
| Source: |
Iranian Journal of Veterinary Medicine; Oct2023, Vol. 17 Issue 4, p393-400, 7p |
| Subject Terms: |
SEMEN analysis; SPERMATOZOA; CRYOPRESERVATION of cells; SPERM motility; ARTIFICIAL insemination; MITOCHONDRIA; ANTIOXIDANTS; FROZEN semen; SEMEN; LIQUID nitrogen |
| Abstract (English): |
Background: Although sperm cryopreservation seems to be an efficient technique for distributing competent sperm for artificial insemination, the process affects the quality of post-thawed sperm. Objectives: This study was designed to see how the novel mitochondria-targeted antioxidant "Mito-TEMPO" affected buck sperm quality during cryopreservation. Methods: After proper semen samples collection, they were diluted and divided into 5 equal groups and cryopreserved in liquid nitrogen with 0, 1, 10, 100, and 1000 μM Mito-TEMPO. Sperm motility, lipid peroxidation, abnormal morphology, acrosome integrity, membrane integrity, and viability were all evaluated after thawing. Results: When the freezing extender was supplemented with 10 μM Mito-TEMPO, total motility, progressive motility, membrane integrity, acrosome integrity, and viability increased (P≤0.05), while lipid peroxidation decreased (P≤0.05). Conclusion: Finally, the novel mitochondria-targeted antioxidant "Mito-TEMPO" could be introduced as an effective cryo-additive to improve buck semen quality parameters during cryopreservation. [ABSTRACT FROM AUTHOR] |
| Abstract (Arabic): |
المقال يركز على تأثير مضاد الأكسدة المستهدف للميتوكوندريا Mito-TEMPO على جودة السائل المنوي بعد التجميد في الماعز. وجدت الدراسة أن إضافة 10 ميكرومول من Mito-TEMPO إلى مادة التجميد المحسنة حسنت بشكل كبير معايير جودة الحيوانات المنوية المختلفة، بما في ذلك الحركة الكلية، الحركة التقدمية، سلامة الغشاء، سلامة الأكروموسوم، والقدرة على الحياة، بينما قللت من أكسدة الدهون. تشير الأبحاث إلى أن Mito-TEMPO يمكن أن يكون مضافًا فعالًا للتجميد لتحسين جودة سائل الماعز المنوي أثناء التجميد، مما يعود بالفائدة على برامج التكاثر. [Extracted from the article] |
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| Database: |
Complementary Index |