| Title: |
Preservation of Buck Semen Quality During Chilling Storage Using Coenzyme Q10. |
| Alternate Title: |
محافظت از کیفیت اسپرم بز در هنگام ذخیره سرمایی با استفاده از کوانزیم کیوتن (Persian) |
| Authors: |
Mohajer, Mokhtar; Asadzadeh, Nader; Barfourooshi, Hoda Javaheri; Davachi, Navid Dadashpour; Masoudi, Reza |
| Source: |
Iranian Journal of Veterinary Medicine; Jul2024, Vol. 18 Issue 3, p429-434, 6p |
| Subject Terms: |
SEMEN analysis; UBIQUINONES; GERM cells; MITOCHONDRIAL physiology; COOLING systems; SPERMATOZOA; FERTILITY; LIPID peroxidation (Biology) |
| Abstract (English): |
Background: Storing spermatozoa in a cold environment decreases spermatozoa metabolism while maintaining sperm fertility. Because of their unique physiological characteristics, sperm cells' reproductive ability in small ruminants is reduced by cooling. Objectives: This study aimed to assess the impact of supplementing chilling medium with coenzyme Q10 (CoQ10) on the quality parameters of buck's semen after chilling at 4°C. Methods: Semen was collected, diluted, and divided into five groups with different CoQ10 supplementation concentrations (0, 1, 2, 5, and 10 μM CoQ10). Collected semen were stored at 4°C for 50 h. The motility, mitochondrial activity, membrane integrity, viability, and lipid peroxidation were measured after 0, 25, and 50 h of chilling. Results: According to the findings, different concentrations of CoQ10 had no impact (P>0.05) on semen at time 0 of storage. After 25 and 50 h of storage, supplementing the chilling medium with 5 μM CoQ10 increased (P≤0.05) progressive motility (PM), total motility (TM), viability, mitochondrial activity, and membrane integrity compared to other groups. Furthermore, 5 μM CoQ10 produced less lipid peroxidation (P≤0.05) after 25 and 50 h than the other groups. Conclusion: Adding 5 μM CoQ10 to the buck's semen cooling medium is an effective procedure for protecting the quality of buck spermatozoa during cooling storage. [ABSTRACT FROM AUTHOR] |
| Abstract (Arabic): |
المقال يركز على الحفاظ على جودة سائل منوي الماعز خلال التخزين في درجات حرارة منخفضة باستخدام الإنزيم المساعد كيو 10 (CoQ10). تقيّم الدراسة تأثيرات تركيزات مختلفة من CoQ10 على معايير جودة متنوعة لسائل منوي الماعز المخزن عند 4 درجات مئوية لمدة تصل إلى 50 ساعة. تشير النتائج إلى أن إضافة 5 ميكرومول من CoQ10 حسّنت بشكل كبير حركة الحيوانات المنوية، والحيوية، والنشاط الميتوكوندري، وسلامة الغشاء، بينما قللت من تأكسد الدهون مقارنةً بتركيزات أخرى. تشير النتائج إلى أن إضافة 5 ميكرومول من CoQ10 إلى وسط التبريد هي طريقة فعالة للحفاظ على جودة الحيوانات المنوية للماعز خلال التخزين. [Extracted from the article] |
| : |
Copyright of Iranian Journal of Veterinary Medicine is the property of University of Tehran and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |