| Title: |
Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment. |
| Authors: |
Rodin, Sergey; Antonsson, Liselotte; Niaudet, Colin; Simonson, Oscar E.; Salmela, Elina; Hansson, Emil M.; Domogatskaya, Anna; Xiao, Zhijie; Damdimopoulou, Pauliina; Sheikhi, Mona; Inzunza, José; Nilsson, Ann-Sofie; Baker, Duncan; Kuiper, Raoul; Sun, Yi; Blennow, Elisabeth; Nordenskjöld, Magnus; Grinnemo, Karl-Henrik; Kere, Juha; Betsholtz, Christer |
| Source: |
Nature Communications; 2/21/2025, Vol. 5 Issue 1, p1-13, 13p |
| Subject Terms: |
STEM cell niches; HUMAN embryonic stem cells; STEM cell culture; MEMBRANE proteins; PLURIPOTENT stem cells; CELL culture |
| Abstract: |
Lack of robust methods for establishment and expansion of pluripotent human embryonic stem (hES) cells still hampers development of cell therapy. Laminins (LN) are a family of highly cell-type specific basement membrane proteins important for cell adhesion, differentiation, migration and phenotype stability. Here we produce and isolate a human recombinant LN-521 isoform and develop a cell culture matrix containing LN-521 and E-cadherin, which both localize to stem cell niches in vivo. This matrix allows clonal derivation, clonal survival and long-term self-renewal of hES cells under completely chemically defined and xeno-free conditions without ROCK inhibitors. Neither LN-521 nor E-cadherin alone enable clonal survival of hES cells. The LN-521/E-cadherin matrix allows hES cell line derivation from blastocyst inner cell mass and single blastomere cells without a need to destroy the embryo. This method can facilitate the generation of hES cell lines for development of different cell types for regenerative medicine purposes. Laminin-511 and its cell-binding domain support long-term self-renewal of human pluripotent stem cells in xeno-free culture conditions. Here the authors show that a xeno-free culture matrix consisting of a full-length laminin-521 and the cell–cell adhesion protein E-cadherin supports clonal expansion of human embryonic stem cells. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |