Diel rhythm of nitrogen and carbon metabolism in the unicellular, diazotrophic cyanobacterium Crocosphaera watsonii WH8501.
| Title: | Diel rhythm of nitrogen and carbon metabolism in the unicellular, diazotrophic cyanobacterium Crocosphaera watsonii WH8501. |
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| Authors: | Mohr W; Leibniz Institute of Marine Sciences (IFM-GEOMAR), Department of Marine Biogeochemistry, Düsternbrooker Weg 20, 24105 Kiel, Germany.; Intermaggio MP; LaRoche J |
| Source: | Environmental microbiology [Environ Microbiol] 2010 Feb; Vol. 12 (2), pp. 412-21. Date of Electronic Publication: 2009 Oct 16. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Blackwell Science Country of Publication: England NLM ID: 100883692 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1462-2920 (Electronic) Linking ISSN: 14622912 NLM ISO Abbreviation: Environ Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Oxford : Blackwell Science, 1999- |
| MeSH Terms: | Carbon/*metabolism ; Cyanobacteria/*metabolism ; Nitrogen/*metabolism; Cyanobacteria/genetics ; Cyanobacteria/physiology ; Nitrogen Fixation/genetics ; Nitrogen Fixation/physiology ; Nitrogenase/genetics ; Nitrogenase/metabolism ; Photosynthesis/genetics ; Animals ; Gene Expression ; Gene Expression Regulation, Bacterial ; Light ; Photoperiod |
| Abstract: | We examined the diel variation in nitrogen and carbon metabolism in Crocosphaera watsonii WH8501 at the physiological and gene expression level in order to determine the temporal constraints for N2 fixation and photosynthesis. N2 fixation and photosynthesis were restricted to the dark and light periods, respectively, during a 24 h light-dark cycle. All genes studied here except one (psbA2) showed diel variations in their expression levels. The highest variation was seen in nifH and nifX relative transcript abundance with a factor of 3-5x10(3) between light and dark periods. Photosynthesis genes showed less variation with a maximum factor of about 500 and always had high relative transcript abundances relative to other genes. At the protein level, the photosystems appeared more stable than the nitrogenase complex over a 24 h light-dark cycle, suggesting that C. watsonii retains the ability to photosynthesize during the dark period of the diel cycle. In contrast, nitrogenase is synthesized daily and exhibits peak abundance during the dark period. Our results have implications for field studies with respect to the interpretation of environmental gene expression data. |
| Substance Nomenclature: | 7440-44-0 (Carbon); EC 1.18.6.1 (Nitrogenase); N762921K75 (Nitrogen) |
| Entry Date(s): | Date Created: 20091021 Date Completed: 20100715 Latest Revision: 20131121 |
| Update Code: | 20260130 |
| DOI: | 10.1111/j.1462-2920.2009.02078.x |
| PMID: | 19840103 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't