Stimulative effect of skipjack tuna soluble extract on pepsin-like protease in the stomach of rockfish (Sebastes schlegelii) using an in vitro perfusion method.
| Title: | Stimulative effect of skipjack tuna soluble extract on pepsin-like protease in the stomach of rockfish (Sebastes schlegelii) using an in vitro perfusion method. |
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| Authors: | Kihara M; Department of Marine Biology and Sciences, Tokai University, Minamisawa 5-1-1-1, Sapporo 005-8601, Japan. kihara@tspirit.tokai-u.jp; Igarashi M; Suzuki T; Itou F; Kozeni S; Toyomane M; Nakano J; Yamai I |
| Source: | Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2011 Apr; Vol. 158 (4), pp. 444-9. Date of Electronic Publication: 2010 Dec 03. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 9806096 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1531-4332 (Electronic) Linking ISSN: 10956433 NLM ISO Abbreviation: Comp Biochem Physiol A Mol Integr Physiol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New York, : Elsevier Science, c1998- |
| MeSH Terms: | Tuna*; Fishes/*metabolism ; Pepsin A/*metabolism ; Stomach/*enzymology; Amino Acids/analysis ; Fishes/anatomy & histology ; Perfusion/methods ; Stomach/anatomy & histology ; Tissue Extracts/chemistry ; Tissue Extracts/pharmacology ; Animals ; Female ; In Vitro Techniques ; Male |
| Abstract: | The effect of intra-gastric infusion of skipjack tuna (Katsuwonus pelamis) muscle soluble extract and of carnosine, anserine and histidine, on pepsin-like protease activity was studied in the isolated, externally batch-cultured stomach of the rockfish (Sebastes schlegelii). Stomach was isolated from the fish, then intragastrically infused with the above solutions or with a balanced saline solution (control solution) as the stimulant at 0.1 mL/min for 6h. Intra-gastric efflux was collected for measurement of pepsin-like protease activity. Skipjack tuna soluble extract, but not carnosine, anserine or histidine alone caused significant enhancement of pepsin-like protease activity during the infusion. Pepsin-like protease activity from skipjack tuna soluble extract responded immediately after infusion and was kept for 150 min after infusion. Stomach motility at the end of infusion was also observed. These results suggest that isolated stomach can receive mucosal side stimulants in vitro. It is likely that some effective components for stomach digestion in fish exist in skipjack tuna soluble extract.; (Copyright © 2010 Elsevier Inc. All rights reserved.) |
| Substance Nomenclature: | 0 (Amino Acids); 0 (Tissue Extracts); EC 3.4.23.1 (Pepsin A) |
| Entry Date(s): | Date Created: 20101207 Date Completed: 20110815 Latest Revision: 20141120 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.cbpa.2010.11.027 |
| PMID: | 21130891 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't