Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity.
| Title: | Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity. |
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| Authors: | Janzen DH; Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.; Hallwachs W; Blandin P; Burns JM; Cadiou JM; Chacon I; Dapkey T; Deans AR; Epstein ME; Espinoza B; Franclemont JG; Haber WA; Hajibabaei M; Hall JP; Hebert PD; Gauld ID; Harvey DJ; Hausmann A; Kitching IJ; Lafontaine D; Landry JF; Lemaire C; Miller JY; Miller JS; Miller L; Miller SE; Montero J; Munroe E; Green SR; Ratnasingham S; Rawlins JE; Robbins RK; Rodriguez JJ; Rougerie R; Sharkey MJ; Smith MA; Solis MA; Sullivan JB; Thiaucourt P; Wahl DB; Weller SJ; Whitfield JB; Willmott KR; Wood DM; Woodley NE; Wilson JJ |
| Source: | Molecular ecology resources [Mol Ecol Resour] 2009 May; Vol. 9 Suppl s1, pp. 1-26. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Blackwell Country of Publication: England NLM ID: 101465604 Publication Model: Print Cited Medium: Print ISSN: 1755-098X (Print) Linking ISSN: 1755098X NLM ISO Abbreviation: Mol Ecol Resour Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Oxford, England : Blackwell |
| Abstract: | Inventory of the caterpillars, their food plants and parasitoids began in 1978 for today's Area de Conservacion Guanacaste (ACG), in northwestern Costa Rica. This complex mosaic of 120 000 ha of conserved and regenerating dry, cloud and rain forest over 0-2000 m elevation contains at least 10 000 species of non-leaf-mining caterpillars used by more than 5000 species of parasitoids. Several hundred thousand specimens of ACG-reared adult Lepidoptera and parasitoids have been intensively and extensively studied morphologically by many taxonomists, including most of the co-authors. DNA barcoding - the use of a standardized short mitochondrial DNA sequence to identify specimens and flush out undisclosed species - was added to the taxonomic identification process in 2003. Barcoding has been found to be extremely accurate during the identification of about 100 000 specimens of about 3500 morphologically defined species of adult moths, butterflies, tachinid flies, and parasitoid wasps. Less than 1% of the species have such similar barcodes that a molecularly based taxonomic identification is impossible. No specimen with a full barcode was misidentified when its barcode was compared with the barcode library. Also as expected from early trials, barcoding a series from all morphologically defined species, and correlating the morphological, ecological and barcode traits, has revealed many hundreds of overlooked presumptive species. Many but not all of these cryptic species can now be distinguished by subtle morphological and/or ecological traits previously ascribed to 'variation' or thought to be insignificant for species-level recognition. Adding DNA barcoding to the inventory has substantially improved the quality and depth of the inventory, and greatly multiplied the number of situations requiring further taxonomic work for resolution.; (© 2009 Blackwell Publishing Ltd and Crown in the right of Canada.) |
| Entry Date(s): | Date Created: 20110514 Date Completed: 20130806 Latest Revision: 20220409 |
| Update Code: | 20260130 |
| DOI: | 10.1111/j.1755-0998.2009.02628.x |
| PMID: | 21564960 |
| Database: | MEDLINE |
Journal Article