Long and short distance movements of β(2)-adrenoceptor in cell membrane assessed by photoconvertible fluorescent protein dendra2-β(2)-adrenoceptor fusion.
| Title: | Long and short distance movements of β(2)-adrenoceptor in cell membrane assessed by photoconvertible fluorescent protein dendra2-β(2)-adrenoceptor fusion. |
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| Authors: | Kaya Aİ; Ankara University Faculty of Medicine, Molecular Biology and Technology Development Unit, 06100 Sıhhiye, Ankara, Turkey.; Uğur O; Altuntaş O; Sayar K; Onaran HO |
| Source: | Biochimica et biophysica acta [Biochim Biophys Acta] 2011 Aug; Vol. 1813 (8), pp. 1511-24. Date of Electronic Publication: 2011 May 19. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Elsevier Pub. Co Country of Publication: Netherlands NLM ID: 0217513 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0006-3002 (Print) Linking ISSN: 00063002 NLM ISO Abbreviation: Biochim Biophys Acta Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam : Elsevier Pub. Co. |
| MeSH Terms: | Cell Membrane/*metabolism ; Receptors, Adrenergic, beta-2/*metabolism; Cyclic AMP/metabolism ; Intracellular Space/metabolism ; Luminescent Proteins/metabolism ; Recombinant Fusion Proteins/metabolism ; Biological Transport, Active ; Diffusion ; Fluorescence Recovery After Photobleaching ; HEK293 Cells ; Humans ; Kinetics ; Microscopy, Confocal ; Models, Biological |
| Abstract: | Local movements of receptors in the plasma membrane have been extensively studied, as it is generally believed that the dynamics of membrane distribution of receptors regulate their functions. However, the properties of large-scale (>5μm) receptor movements in the membrane are relatively obscure. In the present study, we addressed the question as to whether the large-scale movement of receptor in the plasma membrane at the whole cell level can be explained quantitatively by its local diffusive properties. We used HEK 293 cells transfected with human β2-adrenoceptor fused to photoconvertible fluorescent protein dendra2 as a model system; and found that 1) functional integrity of the dendra2-tagged receptor remains apparently intact; 2) in a mesoscopic scale (~4μm), ~90% of the receptors are mobile on average, and receptor influx to, and out-flux from a membrane area can be symmetrically explained by a diffusion-like process with an effective diffusion coefficient of ~0.1μm(2)/s; 3) these mobility parameters are not affected by the activity state of the receptor (assessed by using constitutively active receptor mutants); 4) in the macroscopic scale (4-40μm), although a slowly diffusing fraction of receptors (with D |
| Substance Nomenclature: | 0 (Luminescent Proteins); 0 (Receptors, Adrenergic, beta-2); 0 (Recombinant Fusion Proteins); E0399OZS9N (Cyclic AMP) |
| Entry Date(s): | Date Created: 20110531 Date Completed: 20110830 Latest Revision: 20161126 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.bbamcr.2011.05.008 |
| PMID: | 21621562 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't