Analyzing multiclonality of Staphylococcus aureus in clinical diagnostics using spa-based denaturing gradient gel electrophoresis.
| Title: | Analyzing multiclonality of Staphylococcus aureus in clinical diagnostics using spa-based denaturing gradient gel electrophoresis. |
|---|---|
| Authors: | Matussek A; Department of Laboratory Medicine, Clinical Microbiology, Ryhov County Hospital, Jönköping, Sweden.; Stark L; Dienus O; Aronsson J; Mernelius S; Löfgren S; Lindgren PE |
| Source: | Journal of clinical microbiology [J Clin Microbiol] 2011 Oct; Vol. 49 (10), pp. 3647-8. Date of Electronic Publication: 2011 Aug 10. |
| Publication Type: | Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-660X (Electronic) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, American Society for Microbiology. |
| MeSH Terms: | Coinfection/*microbiology ; Denaturing Gradient Gel Electrophoresis/*methods ; Molecular Typing/*methods ; Staphylococcal Infections/*microbiology ; Staphylococcal Protein A/*genetics ; Staphylococcus aureus/*classification ; Staphylococcus aureus/*genetics; DNA, Bacterial/genetics ; Polymerase Chain Reaction/methods ; Staphylococcus aureus/isolation & purification ; Humans |
| Abstract: | We present a novel denaturing gradient gel electrophoresis (DGGE) method which characterizes multiclonal communities of Staphylococcus aureus. The spa PCR-based DGGE method simultaneously separates strains that differ in only one base, thereby revealing multiclonal colonization and infections. |
| References: | J Clin Microbiol. 2003 Dec;41(12):5442-8. (PMID: 14662923); Environ Microbiol. 2007 Dec;9(12):3134-42. (PMID: 17991040); Clin Microbiol Infect. 2005 Dec;11(12):1027-34. (PMID: 16307559); J Infect Dis. 2005 Feb 1;191(3):444-52. (PMID: 15633104); Proc Natl Acad Sci U S A. 1989 Jan;86(1):232-6. (PMID: 2643100); J Med Microbiol. 2011 Mar;60(Pt 3):317-322. (PMID: 21071544); Am J Infect Control. 2007 Mar;35(2):122-5. (PMID: 17327192); Scand J Infect Dis. 2009;41(6-7):433-9. (PMID: 19431050); J Clin Microbiol. 2006 Sep;44(9):3334-9. (PMID: 16954269); J Clin Microbiol. 2005 Jan;43(1):502-5. (PMID: 15635028); Scand J Infect Dis. 2008;40(11-12):987-9. (PMID: 18759158); Clin Microbiol Rev. 1997 Jul;10(3):505-20. (PMID: 9227864) |
| Substance Nomenclature: | 0 (DNA, Bacterial); 0 (Staphylococcal Protein A) |
| Entry Date(s): | Date Created: 20110812 Date Completed: 20120123 Latest Revision: 20211020 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC3187303 |
| DOI: | 10.1128/JCM.00389-11 |
| PMID: | 21832023 |
| Database: | MEDLINE |
Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't