Live-cell imaging of vesicle trafficking and divalent metal ions by total internal reflection fluorescence (TIRF) microscopy.
| Title: | Live-cell imaging of vesicle trafficking and divalent metal ions by total internal reflection fluorescence (TIRF) microscopy. |
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| Authors: | Loder MK; Section of Cell Biology, Division of Diabetes Endocrinology and Metabolism, Department of Medicine, Faculty of Medicine, Imperial College London, London, UK.; Tsuboi T; Rutter GA |
| Source: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 950, pp. 13-26. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Totowa, NJ : Humana Press; Original Publication: Clifton, N.J. : Humana Press |
| MeSH Terms: | Cations, Divalent/*metabolism ; Imaging, Three-Dimensional/*methods ; Microscopy, Fluorescence/*methods ; Transport Vesicles/*metabolism; Adenoviridae/physiology ; Biological Transport/drug effects ; Biomarkers/metabolism ; Calcium/metabolism ; Cell Survival/drug effects ; Exocytosis/drug effects ; Glucose/pharmacology ; Insulin-Secreting Cells/metabolism ; Insulin-Secreting Cells/virology ; Intracellular Space/drug effects ; Intracellular Space/metabolism ; Neuropeptide Y/metabolism ; Potassium Chloride/pharmacology ; Secretory Vesicles/drug effects ; Secretory Vesicles/metabolism ; Transport Vesicles/drug effects ; Zinc/metabolism ; Animals ; Female ; Humans ; Mice ; Transfection |
| Abstract: | Total internal reflection fluorescence (TIRF) microscopy is an especially powerful tool for visualizing live cellular events. Fluorescent molecules alone provide broad information about the expression and localization of proteins and other molecules; however, the temporal and spatial resolution is confounded by signal from outside the area of interest and the intensity of the illumination required. TIRF overcomes this limitation by using the reflective properties of a laser beam to illuminate a narrow ( |
| Grant Information: | 098424 United Kingdom WT_ Wellcome Trust |
| Substance Nomenclature: | 0 (Biomarkers); 0 (Cations, Divalent); 0 (Neuropeptide Y); 660YQ98I10 (Potassium Chloride); IY9XDZ35W2 (Glucose); J41CSQ7QDS (Zinc); SY7Q814VUP (Calcium) |
| Entry Date(s): | Date Created: 20121023 Date Completed: 20130317 Latest Revision: 20211021 |
| Update Code: | 20260130 |
| DOI: | 10.1007/978-1-62703-137-0_2 |
| PMID: | 23086867 |
| Database: | MEDLINE |
Journal Article