Sclerotic effect of bleomycin on the submandibular gland: an experimental model.
| Title: | Sclerotic effect of bleomycin on the submandibular gland: an experimental model. |
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| Authors: | Güçlü O; Department of Otorhinolaryngology, Canakkale Onsekiz Mart University, Faculty of Medicine, Turkey. droguzguclu@hotmail.com; Muratli A; Arik D; Tekin K; Erdogan H; Dereköy FS |
| Source: | International journal of pediatric otorhinolaryngology [Int J Pediatr Otorhinolaryngol] 2013 Jun; Vol. 77 (6), pp. 943-6. Date of Electronic Publication: 2013 Mar 30. |
| Publication Type: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Elsevier Scientific Publishers Country of Publication: Ireland NLM ID: 8003603 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8464 (Electronic) Linking ISSN: 01655876 NLM ISO Abbreviation: Int J Pediatr Otorhinolaryngol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Limerick : Elsevier Scientific Publishers; Original Publication: [Amsterdam] Elsevier/North-Holland. |
| MeSH Terms: | Bleomycin/*adverse effects ; Sialorrhea/*drug therapy ; Submandibular Gland/*drug effects ; Submandibular Gland/*pathology; Apoptosis/drug effects ; Bleomycin/pharmacology ; Fibrosis/chemically induced ; Fibrosis/pathology ; Sclerosis/chemically induced ; Sclerosis/pathology ; Animals ; Disease Models, Animal ; Female ; Immunohistochemistry ; In Situ Nick-End Labeling ; Male ; Rabbits ; Random Allocation ; Reference Values ; Statistics, Nonparametric |
| Abstract: | Objectives: To evaluate the sclerotic effect of bleomycin on the submandibular gland histopathologically and assess it as a possible alternative therapy for sialorrhea.; Methods: An experimental model was designed and 18 New Zealand white rabbits were used. The rabbits were divided into two groups: a bleomycin group (n=9) and a sham group (n=9). The submandibular glands of the bleomycin group were injected with 0.3 ml bleomycin (3mg/ml) while the sham group received 0.3 ml saline. Four weeks after the procedure, the glands were removed. Histopathological studies including hematoxylin-eosin and Masson's trichrome stain were carried out. The glands were evaluated for tissue inflammation, fibrosis, edema, lipomatosis, atrophy and congestion. To investigate apoptosis, terminal deoxynucleotidyl transferase (TdT)-mediated digoxigenin-11-dUTP nick-end labeling (TUNEL) immunohistochemical staining was used.; Results: In the group injected with bleomycin, inflammation (n=8), edema (n=4), fibrosis (n=3), congestion (n=4) and lipomatosis (n=7) were observed. In the sham group, only lipomatosis was observed. The TUNEL assay results were 5.06 ± 1.18 (p |
| Substance Nomenclature: | 11056-06-7 (Bleomycin) |
| Entry Date(s): | Date Created: 20130404 Date Completed: 20140214 Latest Revision: 20130520 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.ijporl.2013.03.013 |
| PMID: | 23548893 |
| Database: | MEDLINE |
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't