Methods for conjugating antibodies to nanocarriers.
| Title: | Methods for conjugating antibodies to nanocarriers. |
|---|---|
| Authors: | Wagh A; Department of Pharmaceutical Sciences, College of Pharmacy, Nursing and Allied Sciences, North Dakota State University, Fargo, ND, USA.; Law B |
| Source: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 1045, pp. 249-66. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Totowa, NJ : Humana Press; Original Publication: Clifton, N.J. : Humana Press |
| MeSH Terms: | Antibodies/*chemistry ; Drug Carriers/*chemistry ; Nanostructures/*chemistry; Amines/chemistry ; Carbodiimides/chemistry ; Carbohydrates/chemistry ; Carboxylic Acids/chemistry ; Hydrazines/chemistry ; Immunoglobulin G/chemistry ; Periodic Acid/chemistry ; Succinimides/chemistry ; Sulfhydryl Compounds/chemistry ; Oxidation-Reduction |
| Abstract: | Antibodies are one of the most commonly used targeting ligands for nanocarriers, mainly because they are specific, have a strong binding affinity, and are available for a number of disease biomarkers. The bioconjugation chemistry can be a crucial factor in determining the targeting efficiency of drug delivery and should be chosen on a case-by-case basis. An antibody consists of a number of functional groups which offer many flexible options for bioconjugation. This chapter focuses on discussing some of the approaches including periodate oxidation, carbodiimide, maleimide, and heterofunctional linkers, for conjugating antibodies to different nanocarriers. The advantages and limitations are described herein. Specific examples are selected to demonstrate the experimental procedures and to illustrate the potential for applying to other nanocarrier system. |
| Substance Nomenclature: | 0 (Amines); 0 (Antibodies); 0 (Carbodiimides); 0 (Carbohydrates); 0 (Carboxylic Acids); 0 (Drug Carriers); 0 (Hydrazines); 0 (Immunoglobulin G); 0 (Succinimides); 0 (Sulfhydryl Compounds); 10450-60-9 (Periodic Acid); 2OHG8V08NL (N-succinimidyl 3-(2-pyridyldithio)propionate); B45A1BUM4Q (metaperiodate) |
| Entry Date(s): | Date Created: 20130806 Date Completed: 20140217 Latest Revision: 20171116 |
| Update Code: | 20260130 |
| DOI: | 10.1007/978-1-62703-541-5_15 |
| PMID: | 23913152 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't