Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element.
| Title: | Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element. |
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| Authors: | Brzóska K; Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195, Warsaw, Poland, k.brzoska@ichtj.waw.pl.; Stępkowski TM; Kruszewski M |
| Source: | Molecular and cellular biochemistry [Mol Cell Biochem] 2014 Apr; Vol. 389 (1-2), pp. 99-111. Date of Electronic Publication: 2014 Jan 05. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Springer Country of Publication: Netherlands NLM ID: 0364456 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4919 (Electronic) Linking ISSN: 03008177 NLM ISO Abbreviation: Mol Cell Biochem Subsets: MEDLINE |
| Imprint Name(s): | Publication: New York : Springer; Original Publication: The Hague, Dr. W. Junk B. V. Publishers. |
| MeSH Terms: | Antioxidant Response Elements/*genetics ; Carrier Proteins/*genetics ; Gene Expression Regulation/*genetics ; NF-E2-Related Factor 2/*genetics ; Nuclear Proteins/*genetics; NF-kappa B/genetics ; Promoter Regions, Genetic/genetics ; Signal Transduction/genetics ; Transcription Factors/genetics ; Transcription, Genetic/genetics ; Amino Acid Sequence ; Cell Line, Tumor ; Dioxygenases ; HeLa Cells ; Humans ; Molecular Sequence Data ; Sequence Alignment ; Transcription Initiation Site |
| Abstract: | Pirin, a product of the PIR gene, is an iron-binding protein acting as a transcriptional coregulator implicated in the regulation of the NF-κB-related transcription via interaction with RelA (p65), as well as BCL3 and NF-κB1 (p50) proteins. Alterations in pirin expression were observed in various tumors and under oxidative stress conditions. The aim of the present work was to analyze the regulation of the transcription of the human PIR gene. Using constructs containing a different sized PIR promoter and the luciferase reporter genes we found that in HeLa cells PIR transcription is mostly dependent on a highly conserved antioxidant response element located 281 bp downstream of the transcription start site. We have proved that the NRF2 transcription factor binds to this element in vivo and drives the basal PIR expression. We hypothesize that regulation of the PIR expression may constitute a mechanism by which NRF2 is able to modulate the activity of NF-κB and possibly other signaling pathways. |
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| Substance Nomenclature: | 0 (Carrier Proteins); 0 (NF-E2-Related Factor 2); 0 (NF-kappa B); 0 (NFE2L2 protein, human); 0 (Nuclear Proteins); 0 (Transcription Factors); EC 1.13.11.- (Dioxygenases); EC 1.13.11.- (PIR protein, human) |
| Entry Date(s): | Date Created: 20140107 Date Completed: 20141103 Latest Revision: 20211021 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC3950616 |
| DOI: | 10.1007/s11010-013-1931-0 |
| PMID: | 24390086 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't