Association among H. pylori virulence markers dupA, cagA and vacA in Brazilian patients.
| Title: | Association among H. pylori virulence markers dupA, cagA and vacA in Brazilian patients. |
|---|---|
| Authors: | Pereira WN; Ferraz MA; Zabaglia LM; de Labio RW; Orcini WA; Bianchi Ximenez JP; Neto AC; Payão SL; Rasmussen LT; Sacred Heart University, Bauru, São Paulo State, Brazil. lucas_rasmussen@hotmail.com. |
| Source: | The journal of venomous animals and toxins including tropical diseases [J Venom Anim Toxins Incl Trop Dis] 2014 Jan 23; Vol. 20 (1), pp. 1. Date of Electronic Publication: 2014 Jan 23. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: CEVAP Country of Publication: Brazil NLM ID: 101201501 Publication Model: Electronic Cited Medium: Print ISSN: 1678-9199 (Print) Linking ISSN: 16789180 NLM ISO Abbreviation: J Venom Anim Toxins Incl Trop Dis Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Publication: 2019- : Botucatu, SP, Brazil : CEVAP; Original Publication: Botucatu, SP, Brazil : CEVAP, c2003- |
| Abstract: | Background: Only a few Helicobacter pylori-infected individuals develop severe gastric diseases and virulence factors of H. pylori appear to be involved in such clinical outcomes. Duodenal ulcer promoting gene A (dupA) is a novel virulence factor of Helicobacter pylori that is associated with duodenal ulcer development and reduced risk for gastric carcinoma in some populations. The aims of the present study were to determine the presence of dupA gene and evaluate the association among dupA and other virulence factors including cagA and vacA in Brazilian patients. Gastric biopsies were obtained from 205 dyspeptic patients (100 children and 105 adults). DNA was extracted and analyzed for the presence of H. pylori and its virulence factors using the polymerase chain reaction method.; Results: Patients with gastritis tested positive for H. pylori more frequently. The dupA gene was detected in 41.5% of them (85/205); cagA gene was found in 98 isolates (47.8%) and vacA genotype s1/m1 in 50.2%, s1/m2 in 8.3%, s2/m2 in 36.6%, s2/m1 in 0.5% and s1/s2/m1/m2 in 4.4%. We also verified a significant association between cagA and dupA genes [p = 0.0003, relative risk (RR) 1.73 and confidence interval [CI] = 1.3-2.3]. The genotypes s1/m1 were also associated with dupA gene (p = 0.0001, RR: 1.72 and CI: 1.3-2.2). The same associations were found when analyzing pediatric and adult groups of patients individually.; Conclusion: Ours results suggest that dupA is highly frequent in Brazilian patients and is associated with cagA gene and vacA s1/m1 genotype, and it may be considered an important virulence factor in the development of gastric diseases in adults or children. |
| References: | Am J Gastroenterol. 1994 Aug;89(8 Suppl):S116-28. (PMID: 8048402); Gastroenterology. 1997 Jan;112(1):92-9. (PMID: 8978347); Helicobacter. 2007 Dec;12(6):591-7. (PMID: 18001398); J Med Microbiol. 2012 Jan;61(Pt 1):23-30. (PMID: 21903829); Nat Rev Gastroenterol Hepatol. 2010 Nov;7(11):629-41. (PMID: 20938460); Dig Dis Sci. 2010 Jun;55(6):1643-8. (PMID: 19693671); BMC Gastroenterol. 2008 Oct 25;8:49. (PMID: 18950522); Gastroenterol Res Pract. 2012;2012:371503. (PMID: 22829807); J Clin Microbiol. 2013 Mar;51(3):988-9. (PMID: 23254125); Pol J Microbiol. 2012;61(2):147-50. (PMID: 23163215); IARC Monogr Eval Carcinog Risks Hum. 1994;61:1-241. (PMID: 7715068); Int J Med Microbiol. 2008 Apr;298(3-4):223-30. (PMID: 17897881); Int J Med Microbiol. 2011 Mar;301(3):225-8. (PMID: 21050811); Diagn Mol Pathol. 1997 Aug;6(4):238-43. (PMID: 9360845); Microb Pathog. 2013 Jun-Jul;59-60:13-8. (PMID: 23583809); BMC Microbiol. 2012 Jul 02;12:129. (PMID: 22747681); Gastroenterology. 2005 Apr;128(4):833-48. (PMID: 15825067); Clin Exp Med. 2003 Nov;3(3):166-72. (PMID: 14648232); Acta Trop. 2006 Dec;100(3):232-40. (PMID: 17181989); J Clin Microbiol. 1998 May;36(5):1271-6. (PMID: 9574690); J Med Microbiol. 2010 Dec;59(Pt 12):1484-1489. (PMID: 20829397) |
| Entry Date(s): | Date Created: 20140125 Date Completed: 20140213 Latest Revision: 20211021 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC3922733 |
| DOI: | 10.1186/1678-9199-20-1 |
| PMID: | 24456629 |
| Database: | MEDLINE |
Journal Article