Molecular characterisation of lineage IV peste des petits ruminants virus using multi gene sequence data.
| Title: | Molecular characterisation of lineage IV peste des petits ruminants virus using multi gene sequence data. |
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| Authors: | Kumar KS; Tamilnadu Veterinary and Animal Sciences University, Chennai 600051, India.; Babu A; National Institute for Animal Biotechnology, Hyderabad 500049, India.; Sundarapandian G; Tamilnadu Veterinary and Animal Sciences University, Chennai 600051, India.; Roy P; Tamilnadu Veterinary and Animal Sciences University, Chennai 600051, India.; Thangavelu A; Tamilnadu Veterinary and Animal Sciences University, Chennai 600051, India.; Kumar KS; Tamilnadu Veterinary and Animal Sciences University, Chennai 600051, India.; Arumugam R; Tamilnadu Veterinary and Animal Sciences University, Chennai 600051, India.; Chandran ND; Tamilnadu Veterinary and Animal Sciences University, Chennai 600051, India.; Muniraju M; The Pirbright Institute, Ash Road, Woking GU24 0NF, Surrey, United Kingdom.; Mahapatra M; The Pirbright Institute, Ash Road, Woking GU24 0NF, Surrey, United Kingdom.; Banyard AC; Animal Health and Veterinary Laboratories Agency, Woodham Lane, New Haw, Weybridge KT15 3NB, Surrey, United Kingdom.; Manohar BM; Tamilnadu Veterinary and Animal Sciences University, Chennai 600051, India.; Parida S; National Institute for Animal Biotechnology, Hyderabad 500049, India; The Pirbright Institute, Ash Road, Woking GU24 0NF, Surrey, United Kingdom. Electronic address: satya.parida@pirbright.ac.uk. |
| Source: | Veterinary microbiology [Vet Microbiol] 2014 Nov 07; Vol. 174 (1-2), pp. 39-49. Date of Electronic Publication: 2014 Sep 06. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7705469 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2542 (Electronic) Linking ISSN: 03781135 NLM ISO Abbreviation: Vet Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam, Elsevier Scientific Pub. Co. |
| MeSH Terms: | Phylogeny*; Disease Outbreaks/*veterinary ; Goat Diseases/*virology ; Peste-des-Petits-Ruminants/*epidemiology ; Peste-des-Petits-Ruminants/*virology ; Peste-des-petits-ruminants virus/*genetics; DNA Primers/genetics ; Feces/virology ; India/epidemiology ; Peste-des-petits-ruminants virus/classification ; Peste-des-petits-ruminants virus/isolation & purification ; Reverse Transcriptase Polymerase Chain Reaction/veterinary ; Sequence Analysis, DNA/veterinary ; Animals ; Base Sequence ; Cluster Analysis ; Goats ; Molecular Sequence Data |
| Abstract: | Peste des petits ruminants is responsible for an economically important plague of small ruminants that is endemic across much of the developing world. Here we describe the detection and characterisation of a PPR virus from a recent outbreak in Tamil Nadu, India. We demonstrate the isolation of PPR virus from rectal swab and highlight the potential spread of disease to in-contact animals through faecal materials and use of faecal material as non-invasive method of sampling for susceptible wild ruminants. Finally we have performed a comprehensive 'multi-gene' assessment of lineage IV isolates of PPRV utilising sequence data from our study and publically available partial N, partial F and partial H gene data. We describe the effects of grouping PPRV isolates utilising different gene loci and conclude that the variable part of N gene at C terminus gives the best phylogenetic assessment of PPRV isolates with isolates generally clustering according to geographical isolation. This assessment highlights the importance of careful gene targeting with RT-PCR to enable thorough phylogenetic analysis.; (Copyright © 2014 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Grant Information: | BB/H009485/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/L013657/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; HH009485/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/K021400/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/J020478/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/L004801/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council |
| Contributed Indexing: | Keywords: PPR outbreak; Peste des petits ruminants; Phylogenetic analysis using N, F and H genes; RT-PCR |
| Substance Nomenclature: | 0 (DNA Primers) |
| Entry Date(s): | Date Created: 20140925 Date Completed: 20150608 Latest Revision: 20260128 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.vetmic.2014.08.031 |
| PMID: | 25248690 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't