Host candidate gene polymorphisms and associated clearance of P. falciparum amodiaquine and fansidar resistance mutants in children less than 5 years in Cameroon.
| Title: | Host candidate gene polymorphisms and associated clearance of P. falciparum amodiaquine and fansidar resistance mutants in children less than 5 years in Cameroon. |
|---|---|
| Authors: | Ali IM; Evehe MS; Netongo PM; Atogho-Tiedeu B; Akindeh-Nji M; Ngora H; Domkam IK; Diakite M; Baldip K; Ranford-Cartwright L; Mimche PN; Lamb T; Mbacham WF |
| Source: | Pathogens and global health [Pathog Glob Health] 2014 Oct; Vol. 108 (7), pp. 323-33. Date of Electronic Publication: 2014 Nov 12. |
| Publication Type: | Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 101583421 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2047-7732 (Electronic) Linking ISSN: 20477724 NLM ISO Abbreviation: Pathog Glob Health Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2016- : Abingdon : Taylor & Francis; Original Publication: London, UK : Maney Pub. |
| MeSH Terms: | Genetic Predisposition to Disease*; Interleukin-4/*genetics ; Interleukins/*genetics ; Malaria, Falciparum/*genetics ; Malaria, Falciparum/*immunology ; Plasmodium falciparum/*immunology; Amodiaquine/pharmacology ; Antimalarials/pharmacology ; Malaria, Falciparum/parasitology ; Plasmodium falciparum/drug effects ; Pyrimethamine/pharmacology ; Sulfadoxine/pharmacology ; Cameroon ; Child, Preschool ; Drug Combinations ; Drug Resistance ; Female ; Gene Frequency ; Genotype ; Humans ; Infant ; Infant, Newborn ; Male ; Polymorphism, Single Nucleotide ; Interleukin-22 |
| Abstract: | Background: In this post-hoc analysis, we determined the influence of single nucleotide polymorphisms in host candidate immune genes on the outcome of drug resistant malaria in Cameroon.; Methods: Human DNA from 760 patients from a previous clinical trial was subjected to mass spectrometry-based single nucleotide polymorphism (SNP) genotyping. Allele frequencies of candidate immune genes were calculated for 62 SNPs on 17 human chromosomes for their possible involvement in clearance of drug-resistant parasites with the triple mutations of pfcrt76T, pfmdr86Y, and pfmdr1246Y (TY) and pfdhfr51I, pfdhfr59R, pfdhfr108N, and pfdhps437G (IRNG) which were determined by dotblot or PCR-restriction analysis. Differences in SNP frequencies and association analysis were carried out by comparing Chi-square odds ratios (ORs) and stratified by Mantel-Haenzel statistics. An adjusted P value (OR) 39°C for 48 hours) [IL-22, P = 0·01, OR = 1·5, 95% CI (OR): 1·8-2·1] and also in high frequency among the Fulani participants [P = 0·006, OR = 1·83, 95% CI (OR): 1·11-3·08)]. The CD36-1264 null allele was completely absent in the northern population.; Conclusion: Independent association of SNPs in IL22 and IL-4 with clearance of amodiaquine- and sulphadoxine/pyrimethamine-resistant parasites did not reach statistical significance, but may suggest that not all drug-resistant mutants are adversely affected by the same immune-mediated mechanisms of clearance. |
| References: | Nature. 2000 Jun 29;405(6790):1015-6. (PMID: 10890433); J Biol Chem. 2000 Oct 6;275(40):31335-9. (PMID: 10875937); Trans R Soc Trop Med Hyg. 2001 Mar-Apr;95(2):149-52. (PMID: 11355545); Genes Immun. 2001 Nov;2(7):411-4. (PMID: 11704810); J Infect Dis. 2002 Dec 1;186(11):1661-8. (PMID: 12447744); Antimicrob Agents Chemother. 2003 Apr;47(4):1347-54. (PMID: 12654669); J Infect Dis. 2003 Apr 1;187(7):1137-41. (PMID: 12660928); Am J Trop Med Hyg. 2003 Nov;69(5):558-63. (PMID: 14695097); Am J Trop Med Hyg. 2003 Dec;69(6):601-6. (PMID: 14740875); Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5847-51. (PMID: 1631067); Exp Parasitol. 1992 Sep;75(2):207-12. (PMID: 1516668); Bull World Health Organ. 1995;73(5):565-71. (PMID: 8846482); Nucleic Acids Res. 1996 Jul 1;24(13):2623-5. (PMID: 8692708); Am J Hum Genet. 1998 Aug;63(2):498-505. (PMID: 9683598); Infect Immun. 1999 Feb;67(2):513-9. (PMID: 9916053); Drug Resist Updat. 2004 Aug-Oct;7(4-5):233-44. (PMID: 15533761); Am J Trop Med Hyg. 2004 Nov;71(5):531-6. (PMID: 15569778); Indian J Med Res. 2005 Jan;121(1):13-22. (PMID: 15713974); J Exp Med. 2005 Aug 15;202(4):551-60. (PMID: 16087712); Trends Parasitol. 2005 Nov;21(11):494-8. (PMID: 16140578); J Infect Dis. 2006 Apr 1;193(7):978-86. (PMID: 16518760); J Immunol. 2007 Jan 1;178(1):407-15. (PMID: 17182579); Liver Int. 2007 Oct;27(8):1134-43. (PMID: 17845543); Malar J. 2007;6:121. (PMID: 17822535); Am J Trop Med Hyg. 2007 Dec;77(6 Suppl):181-92. (PMID: 18165491); Antimicrob Agents Chemother. 2008 May;52(5):1799-805. (PMID: 18299407); Malar J. 2008;7:125. (PMID: 18613962); Malar J. 2008;7:250. (PMID: 19055786); Immunol Rev. 2008 Dec;226:87-102. (PMID: 19161418); Eur J Immunol. 2009 Mar;39(3):658-61. (PMID: 19283717); Parasite Immunol. 2009 May;31(5):234-53. (PMID: 19388945); Hum Mol Genet. 2009 Jul 15;18(14):2683-92. (PMID: 19403559); Afr Health Sci. 2009 Jun;9(2):66-74. (PMID: 19652739); Malar J. 2010;9:34. (PMID: 20105282); Malar J. 2010;9:119. (PMID: 20459687); PLoS One. 2011;6(9):e23875. (PMID: 21912647); Malar J. 2011;10:271. (PMID: 21929748); Scand J Immunol. 2012 Mar;75(3):355-60. (PMID: 22050542); Cold Spring Harb Protoc. 2012 Mar;2012(3):297-306. (PMID: 22383645); Malar J. 2012;11:109. (PMID: 22480186); Pharm Stat. 2012 Jul-Aug;11(4):300-9. (PMID: 22588983); PLoS One. 2013;8(10):e77214. (PMID: 24130857); Am J Trop Med Hyg. 2014 Oct;91(4):833-43. (PMID: 25048375) |
| Contributed Indexing: | Keywords: Amodiaquine,; Drug resistance markers,; Fever clearance,; Immune response; Interleukin-22,; Parasite clearance,; Sulphadoxine/pyrimethamine, |
| Substance Nomenclature: | 220236ED28 (Amodiaquine); 0 (Antimalarials); 0 (Drug Combinations); 207137-56-2 (Interleukin-4); 0 (Interleukins); Z3614QOX8W (Pyrimethamine); 88463U4SM5 (Sulfadoxine); 0 (Interleukin-22); 0 (IL4 protein, human); 37338-39-9 (fanasil, pyrimethamine drug combination) |
| Entry Date(s): | Date Created: 20141113 Date Completed: 20150701 Latest Revision: 20260128 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC4241784 |
| DOI: | 10.1179/2047773214Y.0000000159 |
| PMID: | 25388906 |
| Database: | MEDLINE |
Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't