Dendritic glycopolymers as dynamic and covalent coating in capillary electrophoresis: view on protein separation processes and detection of nanogram-scaled albumin in biological samples.
| Title: | Dendritic glycopolymers as dynamic and covalent coating in capillary electrophoresis: view on protein separation processes and detection of nanogram-scaled albumin in biological samples. |
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| Authors: | Polikarpov N; Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069 Dresden, Germany(1); Organic Chemistry of Polymers, Technische Universität Dresden, D-01062 Dresden, Germany; Organic Chemistry Department, Saint Petersburg State University, Universitetsky Prospekt 26, Peterhof, 198504 Saint Petersburg, Russia.; Potolytsyna V; Organic Chemistry Department, Saint Petersburg State University, Universitetsky Prospekt 26, Peterhof, 198504 Saint Petersburg, Russia.; Bessonova E; Organic Chemistry Department, Saint Petersburg State University, Universitetsky Prospekt 26, Peterhof, 198504 Saint Petersburg, Russia. Electronic address: bessonova.elena.a@gmail.com.; Tripp S; Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069 Dresden, Germany(1); Organic Chemistry of Polymers, Technische Universität Dresden, D-01062 Dresden, Germany.; Appelhans D; Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069 Dresden, Germany(1).; Voit B; Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069 Dresden, Germany(1); Organic Chemistry of Polymers, Technische Universität Dresden, D-01062 Dresden, Germany.; Kartsova L; Organic Chemistry Department, Saint Petersburg State University, Universitetsky Prospekt 26, Peterhof, 198504 Saint Petersburg, Russia. Electronic address: kartsova@gmail.com. |
| Source: | Journal of chromatography. A [J Chromatogr A] 2015 Jan 23; Vol. 1378, pp. 65-73. Date of Electronic Publication: 2014 Dec 12. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9318488 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3778 (Electronic) Linking ISSN: 00219673 NLM ISO Abbreviation: J Chromatogr A Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam ; New York : Elsevier, 1993- |
| MeSH Terms: | Electrophoresis, Capillary*; Albumins/*analysis ; Chemistry Techniques, Analytical/*methods; Electrolytes/chemistry ; Polymers/chemistry ; Proteins/chemistry ; Limit of Detection |
| Abstract: | Unique properties of dendritic polymers make them promising candidates for application as additives in various analytical methods. From this point, we investigated the potential use of maltose-modified hyperbranched poly(ethylene imine) (PEI-Mal) as a dynamically or covalently bound coating and a pseudostationary phase in capillary electrophoresis. The EOF mobilities were measured at different pH values (2.2, 8.5, and 10.2) using PEI-Mal in the background electrolyte (BGE) and desirable repeatability of the EOF with % RSD (n=50) ≤3.3 was obtained. The influence of pH, polymer concentration, and density of maltose shell on the separation properties of a model mixture of four proteins (albumin, lysozyme, myoglobin, insulin) were investigated. Applying PEI-Mal as a dynamic coating, improved separation of the protein mixture with a high repeatability was achieved. Applying PEI-Mal as a covalent coating for concentrating proteins in the large volume sample stacking (LVSS) combined with the field-enhanced sample injection (FESI), up to 1320-fold enhancement of sensitivity was achieved. The detection limit of 100-500ng/ml allowed successful analysis of albumin level both in blood and urine samples without additional preconcentration.; (Copyright © 2014 Elsevier B.V. All rights reserved.) |
| Contributed Indexing: | Keywords: Capillary electrophoresis; Covalent coating; Dynamic coating; Hyperbranched polymers; On-line concentration; Proteins |
| Substance Nomenclature: | 0 (Albumins); 0 (Electrolytes); 0 (Polymers); 0 (Proteins) |
| Entry Date(s): | Date Created: 20150104 Date Completed: 20150327 Latest Revision: 20181202 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.chroma.2014.11.051 |
| PMID: | 25555410 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't