Innovative approach for urease inhibition by Ficus carica extract-fabricated silver nanoparticles: An in vitro study.
| Title: | Innovative approach for urease inhibition by Ficus carica extract-fabricated silver nanoparticles: An in vitro study. |
|---|---|
| Authors: | Borase HP; School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.; Salunkhe RB; School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.; Patil CD; School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.; Suryawanshi RK; School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.; Salunke BK; School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.; Wagh ND; School of Environment Science, North Maharashtra University, Jalgaon, Maharashtra, India.; Patil SV; School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.; North Maharashtra Microbial Culture Collection Centre (NMCC), North Maharashtra University, Jalgaon, Maharashtra, India. |
| Source: | Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2015 Nov-Dec; Vol. 62 (6), pp. 780-4. Date of Electronic Publication: 2015 Apr 01. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 8609465 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1470-8744 (Electronic) Linking ISSN: 08854513 NLM ISO Abbreviation: Biotechnol Appl Biochem Subsets: MEDLINE |
| Imprint Name(s): | Publication: Jan. 2011- : Malden : Wiley-Blackwell; Original Publication: San Diego : Academic Press, [cl986]- |
| MeSH Terms: | Enzyme Inhibitors/*chemistry ; Enzyme Inhibitors/*pharmacology ; Ficus/*chemistry ; Metal Nanoparticles/*chemistry ; Plant Extracts/*chemistry ; Silver/*chemistry ; Urease/*antagonists & inhibitors; Ammonia/metabolism ; Urease/metabolism |
| Abstract: | In the present study, a rapid, low-cost, and ecofriendly method of stable silver nanoparticles (AgNPs) synthesis using leaves extract of Ficus carica (F. carica), a plant with diverse metabolic consortium, is reported for the first time. An absorption peak at 422 nm in UV-Vis spectroscopy, a spherical shape with an average size of 21 nm in transmission electron microscopy, and crystalline nature in X-ray powder diffraction studies were observed for the synthesized AgNPs. Fourier transform infrared analysis indicated that proteins of F. carica might have a vital role in AgNP synthesis and stabilization. AgNPs were found to inhibit urease, a key enzyme responsible for the survival and pathogenesis of the bacterium, Helicobacter pylori. Inhibition of urease by AgNPs was monitored spectrophotometrically by the evaluation of ammonia release. The urease inhibition potential of AgNPs can be explored in the treatment of H. pylori by preparing novel combinations of standard drugs with AgNPs- or AgNPs-encapsulated drug molecules.; (© 2015 International Union of Biochemistry and Molecular Biology, Inc.) |
| Contributed Indexing: | Keywords: FT-IR; Helicobacter pylori; biomaterials; silver nanoparticles; urease inhibitors |
| Substance Nomenclature: | 0 (Enzyme Inhibitors); 0 (Plant Extracts); 3M4G523W1G (Silver); 7664-41-7 (Ammonia); EC 3.5.1.5 (Urease) |
| Entry Date(s): | Date Created: 20150107 Date Completed: 20161014 Latest Revision: 20220317 |
| Update Code: | 20260130 |
| DOI: | 10.1002/bab.1341 |
| PMID: | 25560197 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't