RE1 silencing transcription factor/neuron-restrictive silencing factor regulates expansion of adult mouse subventricular zone-derived neural stem/progenitor cells in vitro.
| Title: | RE1 silencing transcription factor/neuron-restrictive silencing factor regulates expansion of adult mouse subventricular zone-derived neural stem/progenitor cells in vitro. |
|---|---|
| Authors: | Soldati C; Department of Biology and Biotechnology 'Charles Darwin,' Sapienza University of Rome, Rome, Italy.; Istituto Pasteur Fondazione Cenci Bolognetti, Sapienza University of Rome, Rome, Italy.; Caramanica P; Department of Biology and Biotechnology 'Charles Darwin,' Sapienza University of Rome, Rome, Italy.; Burney MJ; Department of Neuroscience, Centre for the Cellular Basis of Behaviour, Institute of Psychiatry, King's College London, London, United Kingdom.; Toselli C; Department of Biology and Biotechnology 'Charles Darwin,' Sapienza University of Rome, Rome, Italy.; Center for Life Nanoscience Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.; Bithell A; Department of Neuroscience, Centre for the Cellular Basis of Behaviour, Institute of Psychiatry, King's College London, London, United Kingdom.; Augusti-Tocco G; Department of Biology and Biotechnology 'Charles Darwin,' Sapienza University of Rome, Rome, Italy.; Stanton LW; Genome Institute of Singapore, Singapore.; Biagioni S; Department of Biology and Biotechnology 'Charles Darwin,' Sapienza University of Rome, Rome, Italy.; Center for Life Nanoscience Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.; Buckley NJ; Department of Psychiatry, University of Oxford, Warneford Hospital, Oxford, United Kingdom.; Cacci E; Department of Biology and Biotechnology 'Charles Darwin,' Sapienza University of Rome, Rome, Italy.; Istituto Pasteur Fondazione Cenci Bolognetti, Sapienza University of Rome, Rome, Italy. |
| Source: | Journal of neuroscience research [J Neurosci Res] 2015 Aug; Vol. 93 (8), pp. 1203-14. Date of Electronic Publication: 2015 Feb 17. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Wiley Interscience Country of Publication: United States NLM ID: 7600111 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4547 (Electronic) Linking ISSN: 03604012 NLM ISO Abbreviation: J Neurosci Res Subsets: MEDLINE |
| Imprint Name(s): | Publication: New York, NY : Wiley Interscience; Original Publication: New York, Liss. |
| MeSH Terms: | Adult Stem Cells/*physiology ; Gene Silencing/*physiology ; Lateral Ventricles/*cytology ; Lateral Ventricles/*physiology ; Neural Stem Cells/*physiology ; Repressor Proteins/*physiology; Bone Morphogenetic Protein 6/physiology ; Cell Differentiation/physiology ; Cell Proliferation/physiology ; Transcription Factors/physiology ; Animals ; Cells, Cultured ; Humans ; Male ; Mice ; RE1-Silencing Transcription Factor |
| Abstract: | Adult neural stem cell (aNSC) activity is tuned by external stimuli through the recruitment of transcription factors. This study examines the RE1 silencing transcription factor (REST) in neural stem/progenitor cells isolated from the subventricular zone of adult mouse brain and provides the first extensive characterization of REST-mediated control of the cellular and molecular properties. This study shows that REST knockdown affects the capacity of progenitor cells to generate neurospheres, reduces cell proliferation, and triggers cell differentiation despite the presence of growth factors. Genome- and transcriptome-wide analyses show that REST binding sites are significantly enriched in genes associated with synaptic transmission and nervous system development and function. Seeking candidate regulators of aNSC function, this study identifies a member of the bone morphogenetic protein (BMP) family, BMP6, the mRNA and protein of which increased after REST knockdown. The results of this study extend previous findings, demonstrating a reciprocal control of REST expression by BMPs. Administration of exogenous BMP6 inhibits aNSC proliferation and induces the expression of the astrocytic marker glial fibrillary acidic protein, highlighting its antimitogenic and prodifferentiative effects. This study suggests that BMP6 produced in a REST-regulated manner together with other signals can contribute to regulation of NSC maintenance and fate.; (© 2015 Wiley Periodicals, Inc.) |
| Contributed Indexing: | Keywords: REST; bone morphogenetic proteins; cell differentiation; gene expression; neural stem cells; repressor element 1 (RE1)-silencing transcription factor |
| Substance Nomenclature: | 0 (Bone Morphogenetic Protein 6); 0 (Repressor Proteins); 0 (Transcription Factors); 0 (RE1-Silencing Transcription Factor); 0 (Bmp6 protein, mouse) |
| Entry Date(s): | Date Created: 20150219 Date Completed: 20160401 Latest Revision: 20260128 |
| Update Code: | 20260130 |
| DOI: | 10.1002/jnr.23572 |
| PMID: | 25691247 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't