Formation of glutathionyl dinitrosyl iron complexes protects against iron genotoxicity.
| Title: | Formation of glutathionyl dinitrosyl iron complexes protects against iron genotoxicity. |
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| Authors: | Lewandowska H; The Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland. h.lewandowska@ichtj.waw.pl.; Sadło J; Męczyńska S; Stępkowski TM; Wójciuk G; Kruszewski M |
| Source: | Dalton transactions (Cambridge, England : 2003) [Dalton Trans] 2015 Jul 28; Vol. 44 (28), pp. 12640-52. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101176026 Publication Model: Print Cited Medium: Internet ISSN: 1477-9234 (Electronic) Linking ISSN: 14779226 NLM ISO Abbreviation: Dalton Trans Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Cambridge, UK : Royal Society of Chemistry, c2003- |
| MeSH Terms: | DNA Damage/*drug effects ; Glutathione/*pharmacology ; Iron/*pharmacology ; Nitric Oxide Donors/*pharmacology ; Nitrogen Oxides/*pharmacology; DNA/chemistry ; DNA/drug effects ; Glutathione/chemistry ; Hydrogen Peroxide/chemistry ; Iron/chemistry ; Nitric Oxide Donors/chemistry ; Nitrogen Oxides/chemistry ; Circular Dichroism ; Plasmids |
| Abstract: | Dinitrosyl iron(i) complexes (DNICs), intracellular NO donors, are important factors in nitric oxide-dependent regulation of cellular metabolism and signal transduction. It has been shown that NO diminishes the toxicity of iron ions and vice versa. To gain insight into the possible role of DNIC in this phenomenon, we examined the effect of GS-DNIC formation on the ability of iron ions to mediate DNA damage, by treatment of the pUC19 plasmid with physiologically relevant concentrations of GS-DNIC. It was shown that GS-DNIC formation protects against the genotoxic effect of iron ions alone and iron ions in the presence of a naturally abundant antioxidant, GSH. This sheds new light on the iron-related protective effect of NO under the circumstances of oxidative stress. |
| Substance Nomenclature: | 0 (Nitric Oxide Donors); 0 (Nitrogen Oxides); 68586-27-6 (dinitrosyl iron complex); 9007-49-2 (DNA); BBX060AN9V (Hydrogen Peroxide); E1UOL152H7 (Iron); GAN16C9B8O (Glutathione) |
| Entry Date(s): | Date Created: 20150617 Date Completed: 20160426 Latest Revision: 20150708 |
| Update Code: | 20260130 |
| DOI: | 10.1039/c5dt00927h |
| PMID: | 26079708 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't