Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites.
| Title: | Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites. |
|---|---|
| Authors: | Veldkamp CT; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; ‡Department of Biochemistry, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, United States.; Kiermaier E; §IST Austria (Institute for Science and Technology Austria), 3400 Klosterneuburg, Austria.; Gabel-Eissens SJ; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Gillitzer ML; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Lippner DR; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; DiSilvio FA; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Mueller CJ; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Wantuch PL; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Chaffee GR; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Famiglietti MW; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Zgoba DM; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Bailey AA; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Bah Y; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Engebretson SJ; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Graupner DR; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Lackner ER; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; LaRosa VD; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Medeiros T; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Olson ML; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Phillips AJ; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Pyles H; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Richard AM; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Schoeller SJ; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Touzeau B; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Williams LG; †Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, Wisconsin 53190, United States.; Sixt M; §IST Austria (Institute for Science and Technology Austria), 3400 Klosterneuburg, Austria.; Peterson FC; ‡Department of Biochemistry, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, United States. |
| Source: | Biochemistry [Biochemistry] 2015 Jul 14; Vol. 54 (27), pp. 4163-6. Date of Electronic Publication: 2015 Jul 06. |
| Publication Type: | Journal Article; Research Support, N.I.H., Extramural |
| Language: | English |
| Journal Info: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370623 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-4995 (Electronic) Linking ISSN: 00062960 NLM ISO Abbreviation: Biochemistry Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, American Chemical Society. |
| MeSH Terms: | Chemokine CCL19/*chemistry ; Chemokine CCL19/*metabolism ; Membrane Glycoproteins/*metabolism ; Receptors, CCR7/*metabolism; Binding Sites ; Humans ; Models, Molecular ; Protein Conformation |
| Abstract: | CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1's enhancement of resting T-cell recruitment are discussed. |
| References: | Nature. 2001 Mar 1;410(6824):50-6. (PMID: 11242036); Annu Rev Pharmacol Toxicol. 2002;42:469-99. (PMID: 11807180); Biochim Biophys Acta. 2002 Jul 3;1571(3):219-24. (PMID: 12090936); Science. 1997 Nov 14;278(5341):1295-300. (PMID: 9360927); EMBO J. 1997 Dec 1;16(23):6996-7007. (PMID: 9384579); J Immunol Methods. 1999 Feb 1;223(1):77-92. (PMID: 10037236); Eur J Immunol. 2006 Jul;36(7):1659-61. (PMID: 16791884); Nat Immunol. 2007 May;8(5):532-9. (PMID: 17401367); Nat Rev Immunol. 2008 May;8(5):362-71. (PMID: 18379575); Sci Signal. 2008;1(37):ra4. (PMID: 18799424); Protein Expr Purif. 2009 Jun;65(2):251-60. (PMID: 19297698); Immunol Rev. 2009 Jul;230(1):75-96. (PMID: 19594630); Proc Natl Acad Sci U S A. 2010 Sep 28;107(39):16934-9. (PMID: 20837531); Methods Mol Biol. 2011;769:149-65. (PMID: 21748675); Retrovirology. 2011;8:80. (PMID: 21992606); Biochemistry. 2012 Jan 24;51(3):733-5. (PMID: 22221265); Science. 2013 Jan 18;339(6117):328-32. (PMID: 23329049); Blood. 2013 May 30;121(22):4484-92. (PMID: 23610371); Nat Rev Immunol. 2013 Nov;13(11):815-29. (PMID: 24319779); Br J Pharmacol. 2014 Mar;171(5):1167-79. (PMID: 24116930); Int J Biochem Cell Biol. 2014 Sep;54:78-82. (PMID: 25019368); Immunol Cell Biol. 2015 Apr;93(4):372-83. (PMID: 25708536) |
| Grant Information: | 281556 International ERC_ European Research Council; R15 CA159202 United States CA NCI NIH HHS; 1R15CA159202-01 United States CA NCI NIH HHS |
| Molecular Sequence: | PDB 2MP1 |
| Substance Nomenclature: | 0 (CCR7 protein, human); 0 (Chemokine CCL19); 0 (Membrane Glycoproteins); 0 (P-selectin ligand protein); 0 (Receptors, CCR7) |
| Entry Date(s): | Date Created: 20150627 Date Completed: 20151001 Latest Revision: 20250529 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC4809050 |
| DOI: | 10.1021/acs.biochem.5b00560 |
| PMID: | 26115234 |
| Database: | MEDLINE |
Journal Article; Research Support, N.I.H., Extramural