Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum.
| Title: | Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum. |
|---|---|
| Authors: | Leßmeier L; Genetics of Prokaryotes, Faculty of Biology & Center for Biotechnology, Bielefeld University, Bielefeld, 33615, Germany. lennart@cebitec.uni-bielefeld.de.; Wendisch VF; Genetics of Prokaryotes, Faculty of Biology & Center for Biotechnology, Bielefeld University, Bielefeld, 33615, Germany. volker.wendisch@uni-bielefeld.de. |
| Source: | BMC microbiology [BMC Microbiol] 2015 Oct 16; Vol. 15, pp. 216. Date of Electronic Publication: 2015 Oct 16. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : BioMed Central, [2001- |
| MeSH Terms: | Drug Tolerance* ; Point Mutation*; Corynebacterium glutamicum/*drug effects ; Corynebacterium glutamicum/*genetics ; DNA, Bacterial/*chemistry ; DNA, Bacterial/*genetics ; Methanol/*toxicity; Carbon-Oxygen Lyases/genetics ; Coenzyme A-Transferases/genetics ; Corynebacterium glutamicum/growth & development ; Genome, Bacterial ; Reverse Genetics ; Selection, Genetic ; Sequence Analysis, DNA ; Serial Passage |
| Abstract: | Background: Methanol is present in most ecosystems and may also occur in industrial applications, e.g. as an impurity of carbon sources such as technical glycerol. Methanol often inhibits growth of bacteria, thus, methanol tolerance may limit fermentative production processes.; Results: The methanol tolerance of the amino acid producing soil bacterium Corynebacterium glutamicum was improved by experimental evolution in the presence of methanol. The resulting strain Tol1 exhibited significantly increased growth rates in the presence of up to 1 M methanol. However, neither transcriptional changes nor increased enzyme activities of the linear methanol oxidation pathway were observed, which was in accordance with the finding that tolerance to the downstream metabolites formaldehyde and formate was not improved. Genome sequence analysis of strain Tol1 revealed two point mutations potentially relevant to enhanced methanol tolerance: one leading to the amino acid exchange A165T of O-acetylhomoserine sulfhydrolase MetY and the other leading to shortened CoA transferase Cat (Q342*). Introduction of either mutation into the genome of C. glutamicum wild type increased methanol tolerance and introduction of both mutations into C. glutamicum was sufficient to achieve methanol tolerance almost indistinguishable from that of strain Tol1.; Conclusion: The methanol tolerance of C. glutamicum can be increased by two point mutations leading to amino acid exchange of O-acetylhomoserine sulfhydrolase MetY and shortened CoA transferase Cat. Introduction of these mutations into producer strains may be helpful when using carbon sources containing methanol as component or impurity. |
| References: | Appl Environ Microbiol. 2015 Mar;81(6):2215-25. (PMID: 25595770); Appl Microbiol Biotechnol. 2015 Dec;99(23):10163-76. (PMID: 26276544); J Bacteriol. 1993 Sep;175(17):5595-603. (PMID: 8366043); Gene. 1994 Jul 22;145(1):69-73. (PMID: 8045426); Microbiology. 1994 Aug;140 ( Pt 8):1817-28. (PMID: 7522844); Arch Microbiol. 1996 Jun;165(6):387-96. (PMID: 8661932); Arch Microbiol. 1997 Oct;168(4):262-9. (PMID: 9297462); Microbiology. 1999 Feb;145 ( Pt 2):503-13. (PMID: 10075432); J Occup Health. 2006 Jan;48(1):20-7. (PMID: 16484759); J Bacteriol. 2006 Apr;188(7):2554-67. (PMID: 16547043); J Bacteriol. 2006 Apr;188(8):3063-72. (PMID: 16585766); Plant J. 2006 Jun;46(6):948-60. (PMID: 16805729); J Mol Microbiol Biotechnol. 2007;12(1-2):51-9. (PMID: 17183211); Appl Microbiol Biotechnol. 2007 Feb;74(1):22-34. (PMID: 17216461); FEMS Microbiol Lett. 2007 Aug;273(1):109-19. (PMID: 17559405); Appl Microbiol Biotechnol. 2007 Oct;76(6):1347-56. (PMID: 17646983); J Bacteriol. 2007 Oct;189(20):7408-16. (PMID: 17693518); J Microbiol Biotechnol. 2007 Jun;17(6):1010-7. (PMID: 18050920); J Mol Microbiol Biotechnol. 2008;15(4):222-33. (PMID: 17693703); Appl Environ Microbiol. 2008 Oct;74(20):6216-22. (PMID: 18757581); J Pharm Pharmacol. 2000 May;52(5):547-52. (PMID: 10864143); Chem Biol Interact. 2001 Jan 30;130-132(1-3):285-96. (PMID: 11306052); J Mol Microbiol Biotechnol. 2001 Apr;3(2):295-300. (PMID: 11321586); Trends Biotechnol. 2009 Feb;27(2):107-15. (PMID: 19111927); J Biotechnol. 2009 Mar 10;140(1-2):84-91. (PMID: 19041911); J Biotechnol. 2009 Mar 10;140(1-2):75-83. (PMID: 19162097); FEMS Microbiol Lett. 2010 Mar;304(2):107-15. (PMID: 20377641); J Microbiol Biotechnol. 2010 Aug;20(8):1196-203. (PMID: 20798582); Nucleic Acids Res. 2011 Jan;39(Database issue):D225-9. (PMID: 21109532); Bioinformatics. 2011 May 15;27(10):1351-8. (PMID: 21450712); Appl Microbiol Biotechnol. 2012 Jul;95(1):13-27. (PMID: 22581036); Microbiology. 2012 Sep;158(Pt 9):2428-39. (PMID: 22767548); J Bacteriol. 2013 Apr;195(8):1718-26. (PMID: 23396909); Appl Environ Microbiol. 2013 Sep;79(17):5313-20. (PMID: 23811509); Bioresour Technol. 2013 Oct;145:254-8. (PMID: 23562176); Appl Environ Microbiol. 2013 Oct;79(19):6006-15. (PMID: 23892752); PLoS One. 2013;8(9):e76579. (PMID: 24086751); Appl Environ Microbiol. 2013 Nov;79(22):6974-83. (PMID: 24014532); Microbiology. 2013 Dec;159(Pt 12):2651-62. (PMID: 24065717); Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20034-9. (PMID: 24277830); J Biosci Bioeng. 2014 Feb;117(2):197-9. (PMID: 23916855); Curr Opin Biotechnol. 2014 Apr;26:38-44. (PMID: 24679256); Curr Opin Biotechnol. 2014 Dec;30:51-8. (PMID: 24922334); J Biotechnol. 2014 Nov 20;190:40-54. (PMID: 25107507); Microb Biotechnol. 2015 Mar;8(2):342-50. (PMID: 25644214); Appl Environ Microbiol. 2015 Mar;81(6):1996-2005. (PMID: 25576602); Biochemistry. 2001 Aug 21;40(33):9799-809. (PMID: 11502173); Nucleic Acids Res. 2002 Jan 1;30(1):207-10. (PMID: 11752295); Microbiology. 2002 Jan;148(Pt 1):333-40. (PMID: 11782526); J Bacteriol. 2002 Mar;184(5):1277-86. (PMID: 11844756); J Bacteriol. 1977 Apr;130(1):62-73. (PMID: 15989); Biochemistry. 1983 Apr 12;22(8):1857-63. (PMID: 6342669); J Mol Biol. 1983 Jun 5;166(4):557-80. (PMID: 6345791); Life Sci. 1991;48(11):1031-41. (PMID: 1997785); Pharmacol Toxicol. 1991 Sep;69(3):157-63. (PMID: 1665561); Regul Toxicol Pharmacol. 1992 Oct;16(2):150-60. (PMID: 1438995); J Bacteriol. 2002 May;184(10):2728-39. (PMID: 11976302); Eur J Biochem. 2002 Apr;269(8):2117-23. (PMID: 11985589); Arch Microbiol. 2002 Oct;178(4):239-49. (PMID: 12209256); Curr Microbiol. 2002 Nov;45(5):362-7. (PMID: 12232668); J Biotechnol. 2003 Sep 4;104(1-3):5-25. (PMID: 12948626); J Biotechnol. 2003 Sep 4;104(1-3):273-85. (PMID: 12948645); J Bacteriol. 2004 May;186(9):2798-809. (PMID: 15090522); Biochim Biophys Acta. 1976 Apr 9;430(1):13-29. (PMID: 4141); Br J Ind Med. 1985 Sep;42(9):591-5. (PMID: 3899160) |
| Substance Nomenclature: | 0 (DNA, Bacterial); EC 2.5.1.47 (O-acetylhomoserine (thiol)-lyase); EC 2.8.3.- (Coenzyme A-Transferases); EC 4.2.- (Carbon-Oxygen Lyases); Y4S76JWI15 (Methanol) |
| Entry Date(s): | Date Created: 20151018 Date Completed: 20160628 Latest Revision: 20181113 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC4609165 |
| DOI: | 10.1186/s12866-015-0558-6 |
| PMID: | 26474849 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't