The mystery of the fourth clone: comparative genomic analysis of four non-typeable Streptococcus pneumoniae strains with different susceptibilities to optochin.
| Title: | The mystery of the fourth clone: comparative genomic analysis of four non-typeable Streptococcus pneumoniae strains with different susceptibilities to optochin. |
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| Authors: | Ikryannikova LN; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia. Larisa.Ikryannikova@gmail.com.; Ischenko DS; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia.; Lominadze GG; Federal State Budgetary Institute 'Scientific Center of Children Health' of RAMS, Moscow, Russia.; Kanygina AV; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia.; Karpova IY; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia.; Kostryukova ES; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia.; Mayansky NA; Federal State Budgetary Institute 'Scientific Center of Children Health' of RAMS, Moscow, Russia.; Skvortsov VS; Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121, Russia.; Ilina EN; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia.; Govorun VM; Research Institute of Physical-Chemical Medicine, Ministry of Public Health of Russian Federation, 119992, Malaya Pirogovskaya str., 1a, Moscow, Russia. |
| Source: | European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2016 Jan; Vol. 35 (1), pp. 119-30. Date of Electronic Publication: 2015 Nov 12. |
| Publication Type: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Springer Country of Publication: Germany NLM ID: 8804297 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-4373 (Electronic) Linking ISSN: 09349723 NLM ISO Abbreviation: Eur J Clin Microbiol Infect Dis Subsets: MEDLINE |
| Imprint Name(s): | Publication: Berlin : Springer; Original Publication: [Wiesbaden, Federal Republic of Germany] : Vieweg, [c1988- |
| MeSH Terms: | Drug Resistance, Bacterial* ; Genome, Bacterial* ; Genomics*; Anti-Infective Agents/*pharmacology ; Quinine/*analogs & derivatives ; Streptococcus pneumoniae/*drug effects ; Streptococcus pneumoniae/*genetics; Mitochondrial Proton-Translocating ATPases/chemistry ; Mitochondrial Proton-Translocating ATPases/metabolism ; Pneumococcal Infections/microbiology ; Quinine/pharmacology ; Streptococcus pneumoniae/isolation & purification ; Humans ; Microbial Sensitivity Tests ; Molecular Dynamics Simulation ; Mutation, Missense ; Protein Binding ; Sequence Analysis, DNA |
| Abstract: | Optochin-resistant pneumococci can be rarely caught in clinical microbiology laboratories because of the routine identification of all such strains as viridans group non-pneumococci. We were lucky to find four non-typeable Streptococcus pneumoniae clones demonstrating the different susceptibilities to optochin: one of them (Spn_13856) was resistant to optochin, while the other three (Spn_1719, Spn_27, and Spn_2298) were susceptible. Whole genome nucleotide sequences of these strains were compared to reveal the differences between the optochin-resistant and optochin-susceptible strains. Two adjacent genes coding maltose O-acetyltransferase and uridine phosphorylase which were presented in the genomes of all optochin-susceptible strains and missed in the optochin-resistant strain were revealed. Non-synonymous substitutions in 14 protein-coding genes were discovered, including the Ala49Ser mutation in the C-subunit of the F0 part of the ATP synthase rotor usually associated with pneumococcal optochin resistance. Modeling of a process of optochin interaction with the F0 part of the ATP synthase rotor indicates that the complex of optochin with "domain C" composed by wild-type C-subunits is more stable than the same complex composed of Ala49Ser mutant C-subunits. |
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| Substance Nomenclature: | 0 (Anti-Infective Agents); 4W030VS2OG (ethylhydrocupreine); A7V27PHC7A (Quinine); EC 3.6.1.- (F1F0-ATP synthase); EC 3.6.3.- (Mitochondrial Proton-Translocating ATPases) |
| Entry Date(s): | Date Created: 20151114 Date Completed: 20161114 Latest Revision: 20181113 |
| Update Code: | 20260130 |
| DOI: | 10.1007/s10096-015-2516-5 |
| PMID: | 26563895 |
| Database: | MEDLINE |
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't