Downregulated Expression of E-cadherin and TP53 in Patients with Gastric Diseases: the Involvement of H. pylori Infection and Its Virulence Markers.
| Title: | Downregulated Expression of E-cadherin and TP53 in Patients with Gastric Diseases: the Involvement of H. pylori Infection and Its Virulence Markers. |
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| Authors: | Ferraz MA; Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil.; Zabaglia LM; Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil.; Pereira WN; Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil.; Orcini WA; Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil.; de Labio RW; Departamento de Genética e Biologia Molecular, Hemocentro, Faculdade de Medicina de Marília, Rua Monte Carmelo, 800, Marília, 17519050, Brazil.; Caleman Neto A; Departamento de Genética e Biologia Molecular, Hemocentro, Faculdade de Medicina de Marília, Rua Monte Carmelo, 800, Marília, 17519050, Brazil.; Wisnieski F; Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023900, Brazil.; Calcagno DQ; Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023900, Brazil.; Santos LC; Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023900, Brazil.; Assumpção PP; Núcleo de Pesquisa em Oncologia, Hospital João de Barros Barreto, Universidade Federal do Pará, Avenida Mundurucus, 4487, Belém, 66073000, Brazil.; Burbano RR; Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Correia, 01, Belém, 66075110, Brazil.; Artigiani R; Departamento de Patologia, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023000, Brazil.; Smith Mde A; Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023900, Brazil.; Payão SL; Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil.; Rasmussen LT; Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil. lucasrasmussen@gmail.com. |
| Source: | Journal of gastrointestinal cancer [J Gastrointest Cancer] 2016 Mar; Vol. 47 (1), pp. 20-6. |
| Publication Type: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Springer Country of Publication: United States NLM ID: 101479627 Publication Model: Print Cited Medium: Internet ISSN: 1941-6636 (Electronic) NLM ISO Abbreviation: J Gastrointest Cancer Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2010- : New York : Springer; Original Publication: New York, NY : Humana Press |
| MeSH Terms: | Antigens, Bacterial/*genetics ; Bacterial Proteins/*genetics ; Biomarkers/*analysis ; Cadherins/*genetics ; Helicobacter Infections/*genetics ; Stomach Neoplasms/*genetics ; Tumor Suppressor Protein p53/*genetics ; Virulence/*genetics; DNA, Viral/genetics ; Gastric Mucosa/metabolism ; Gastric Mucosa/pathology ; Gastritis/genetics ; Gastritis/pathology ; Gastritis/virology ; Helicobacter Infections/metabolism ; Helicobacter Infections/pathology ; Helicobacter Infections/virology ; RNA, Messenger/genetics ; Stomach Neoplasms/pathology ; Stomach Neoplasms/virology ; Adult ; Case-Control Studies ; Down-Regulation ; Female ; Follow-Up Studies ; Helicobacter pylori ; Humans ; Male ; Neoplasm Staging ; Prognosis ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Survival Rate |
| Abstract: | Purpose: Gastritis caused by infection with Helicobacter pylori is characterized by chronic inflammation and damage in gastric tissue, which is a main risk factor for gastric cancer. Associated with H. pylori, the TP53 gene tumor suppressor and the cell adhesion glycoprotein epithelial cadherin develop a relevant role in the integrity and carcinogenesis of the epithelium. We aimed to detection of H. pylori and its main virulence markers and measured the messenger RNA (mRNA) expression levels of E-cadherin and TP53 genes.; Methods: The detection of H. pylori and its virulence markers, as well as the mRNA expression levels of E-cadherin and TP53 genes, were obtained for 161 samples of gastric biopsies including 37 with normal gastric tissue, 70 with gastritis, 24 from neoplastic tissue, and 27 from adjacent non-neoplastic by means of a quantitative real-time polymerase chain reaction.; Results: The mRNA expression levels of E-cadherin and TP53 were found to be decreased in patients with gastritis, independently of H. pylori infection. In samples from gastric patients, the neoplastic tissue showed an accentuated decrease of expression; on the other hand, the expression of E-cadherin was normal in adjacent non-neoplastic.; Conclusions: No evidence was found of the involvement of the cagA and vacA genes in the decreased expression of E-cadherin and TP53. The process of carcinogenesis is complex, and the decrease of the E-cadherin gene expression and TP53 gene expression appears to contribute significantly. |
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| Contributed Indexing: | Keywords: E-cadherin; Gastric cancer; Gene expression; Helicobacter pylori; TP53 |
| Substance Nomenclature: | 0 (Antigens, Bacterial); 0 (Bacterial Proteins); 0 (Biomarkers); 0 (Cadherins); 0 (DNA, Viral); 0 (RNA, Messenger); 0 (TP53 protein, human); 0 (Tumor Suppressor Protein p53); 0 (VacA protein, Helicobacter pylori); 0 (cagA protein, Helicobacter pylori) |
| Entry Date(s): | Date Created: 20151121 Date Completed: 20161213 Latest Revision: 20181113 |
| Update Code: | 20260130 |
| DOI: | 10.1007/s12029-015-9782-4 |
| PMID: | 26585943 |
| Database: | MEDLINE |
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't