Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori.
| Title: | Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori. |
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| Authors: | Zabaglia LM; Universidade Sagrado Coração, Rua Irmã Arminda 10-50, Jardim Brasil, CEP 17011-160 Bauru, SP Brazil.; Ferraz MA; Universidade Sagrado Coração, Rua Irmã Arminda 10-50, Jardim Brasil, CEP 17011-160 Bauru, SP Brazil.; Pereira WN; Universidade Sagrado Coração, Rua Irmã Arminda 10-50, Jardim Brasil, CEP 17011-160 Bauru, SP Brazil.; Orcini WA; Universidade Sagrado Coração, Rua Irmã Arminda 10-50, Jardim Brasil, CEP 17011-160 Bauru, SP Brazil.; de Labio RW; Faculdade de Medicina de Marília, Rua Lourival Freire 240, 17519-050 Marília, SP Brazil.; Neto AC; Faculdade de Medicina de Marília, Rua Lourival Freire 240, 17519-050 Marília, SP Brazil.; Wisnieski F; Universidade Federal de São Paulo, Rua Sena Madureira, 1500, 04021-001 São Paulo, SP Brazil.; de Oliveira JG; Universidade Sagrado Coração, Rua Irmã Arminda 10-50, Jardim Brasil, CEP 17011-160 Bauru, SP Brazil.; de Arruda Cardoso Smith M; Universidade Federal de São Paulo, Rua Sena Madureira, 1500, 04021-001 São Paulo, SP Brazil.; Payão SL; Universidade Sagrado Coração, Rua Irmã Arminda 10-50, Jardim Brasil, CEP 17011-160 Bauru, SP Brazil ; Faculdade de Medicina de Marília, Rua Lourival Freire 240, 17519-050 Marília, SP Brazil.; Rasmussen LT; Universidade Sagrado Coração, Rua Irmã Arminda 10-50, Jardim Brasil, CEP 17011-160 Bauru, SP Brazil. |
| Source: | The journal of venomous animals and toxins including tropical diseases [J Venom Anim Toxins Incl Trop Dis] 2015 Dec 30; Vol. 21, pp. 54. Date of Electronic Publication: 2015 Dec 30 (Print Publication: 2015). |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: CEVAP Country of Publication: Brazil NLM ID: 101201501 Publication Model: eCollection Cited Medium: Print ISSN: 1678-9199 (Print) Linking ISSN: 16789180 NLM ISO Abbreviation: J Venom Anim Toxins Incl Trop Dis Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Publication: 2019- : Botucatu, SP, Brazil : CEVAP; Original Publication: Botucatu, SP, Brazil : CEVAP, c2003- |
| Abstract: | Background: Tumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may be influenced by -308 (G > A) polymorphisms and gene expression of the TNF-α gene.; Methods: One hundred and thirty-four gastric biopsy samples were collected from patients of both genders (61♂ and 73♀, mean age 40.3 ± 24.2 years) with gastric symptoms. The -308 (G > A) polymorphism of TNF-α was characterized using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The expression level was measured using real-time PCR, and relative quantification (RQ) was calculated using the comparative CT method (2(-ΔΔCT)).; Results: The analysis revealed an increase in TNF-α gene expression in patients with gastritis; on the other hand, no statistical differences were observed in patients with gastric cancer. In addition, no association was found among -308 polymorphism genotypes, virulence markers, or TNF-α gene expression.; Conclusions: Helicobacter pylori induces a large increase in TNF-α expression in patients with gastritis, regardless of tissue inflammation, but after the tissue becomes neoplastic, the presence of bacteria did not influence expression. These results suggest that the TNF-α pathway may play an important role in the progression from gastritis to gastric cancer. |
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| Contributed Indexing: | Keywords: Gastric cancer; Gene expression; H. pylori; TNF-α; cagA |
| Entry Date(s): | Date Created: 20160101 Date Completed: 20151231 Latest Revision: 20201001 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC4696262 |
| DOI: | 10.1186/s40409-015-0054-3 |
| PMID: | 26719751 |
| Database: | MEDLINE |
Journal Article