Modelling affective pain in mice: Effects of inflammatory hypersensitivity on place escape/avoidance behaviour, anxiety and hedonic state.
| Title: | Modelling affective pain in mice: Effects of inflammatory hypersensitivity on place escape/avoidance behaviour, anxiety and hedonic state. |
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| Authors: | Refsgaard LK; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Jagtvej 160, DK-2100 Copenhagen, Denmark. Electronic address: louiserefsgaard@hotmail.com.; Hoffmann-Petersen J; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Jagtvej 160, DK-2100 Copenhagen, Denmark.; Sahlholt M; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Jagtvej 160, DK-2100 Copenhagen, Denmark.; Pickering DS; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Jagtvej 160, DK-2100 Copenhagen, Denmark.; Andreasen JT; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Jagtvej 160, DK-2100 Copenhagen, Denmark. |
| Source: | Journal of neuroscience methods [J Neurosci Methods] 2016 Mar 15; Vol. 262, pp. 85-92. Date of Electronic Publication: 2016 Jan 25. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7905558 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-678X (Electronic) Linking ISSN: 01650270 NLM ISO Abbreviation: J Neurosci Methods Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| MeSH Terms: | Pain*/diagnosis ; Pain*/etiology ; Pain*/psychology ; Disease Models, Animal*; Anxiety Disorders/*etiology ; Avoidance Learning/*physiology ; Inflammation/*complications; Anxiety Disorders/diagnosis ; Freund's Adjuvant/toxicity ; Hyperalgesia/physiopathology ; Inflammation/chemically induced ; Locomotion/drug effects ; Locomotion/physiology ; Maze Learning/physiology ; Pain Threshold/physiology ; Saccharin/administration & dosage ; Sweetening Agents/administration & dosage ; Analysis of Variance ; Animals ; Female ; Food Preferences ; Mice ; Mice, Inbred C57BL ; Pain Measurement ; Time Factors |
| Abstract: | Background: The place escape/avoidance paradigm (PEAP) has been used to assess the affective component of pain in rats. Using the Complete Freund's Adjuvant (CFA) model of inflammatory pain, the current study aimed at developing a mouse version of PEAP and investigating the relation between PEAP and other behavioural responses, namely anxiety-like behaviour, locomotor activity, and hedonic state.; New Method: A novel paradigm assessing the affective component of pain in mice was developed by modifying the setup known from rat studies: Animals were forced to stay 2 × 5 min in the light and the dark area of a box while being stimulated with a suprathreshold filament on the untreated or treated paw, respectively. This was followed by a 30-min test with unrestricted movement. Anxiety-like behaviour, locomotor activity, and hedonic state were assessed with the elevated zero maze (EZM), an open field setup, and a saccharin preference test, respectively, and correlated with the PEAP behaviour to examine potentially confounding parameters of the novel paradigm.; Results: In the PEAP, CFA-treated animals spent more time in the light area. CFA also increased anxiety-like behaviour significantly, whereas locomotor activity was unaffected. A significant, albeit modest, reduction in saccharin preference was observed. PEAP responses showed no significant correlations with any other behavioural measure.; Comparison With Existing Method and Conclusions: The PEAP results suggest that this paradigm might be successfully applied in mice to study affective pain. CFA treatment was associated with increased anxiety-like behaviour and anhedonia; however, this appeared unrelated to the PEAP responses.; (Copyright © 2016 Elsevier B.V. All rights reserved.) |
| Contributed Indexing: | Keywords: Affective pain; Anhedonia; Anxiety; Avoidance behaviour; CFA |
| Substance Nomenclature: | 0 (Sweetening Agents); 9007-81-2 (Freund's Adjuvant); FST467XS7D (Saccharin) |
| Entry Date(s): | Date Created: 20160129 Date Completed: 20161213 Latest Revision: 20161230 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.jneumeth.2016.01.019 |
| PMID: | 26820902 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't