Rapid and selective detection of experimental snake envenomation - Use of gold nanoparticle based lateral flow assay.
| Title: | Rapid and selective detection of experimental snake envenomation - Use of gold nanoparticle based lateral flow assay. |
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| Authors: | Pawade BS; Department of Antitoxins & Sera, Haffkine Biopharmaceutical Corporation Ltd., Pune, India. Electronic address: rajapawade@yahoo.co.in.; Salvi NC; Department of Antitoxins & Sera, Haffkine Biopharmaceutical Corporation Ltd., Pune, India.; Shaikh IK; Department of Antitoxins & Sera, Haffkine Biopharmaceutical Corporation Ltd., Pune, India.; Waghmare AB; Department of Antitoxins & Sera, Haffkine Biopharmaceutical Corporation Ltd., Pune, India.; Jadhav ND; Department of Antitoxins & Sera, Haffkine Biopharmaceutical Corporation Ltd., Pune, India.; Wagh VB; Department of Microbiology, MGM Medical College, Navi Mumbai, India.; Pawade AS; Department of Pharmacognosy, Marathwada Mitra Mandal's College of Pharmacy, Pune, India.; Waykar IG; Department of Antitoxins & Sera, Haffkine Biopharmaceutical Corporation Ltd., Pune, India.; Potnis-Lele M; Entrepreneurship Development Centre, 100 NCL Innovation Park Campus, Pashan, Pune, India. |
| Source: | Toxicon : official journal of the International Society on Toxinology [Toxicon] 2016 Sep 01; Vol. 119, pp. 299-306. Date of Electronic Publication: 2016 Jul 01. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Pergamon Press Country of Publication: England NLM ID: 1307333 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3150 (Electronic) Linking ISSN: 00410101 NLM ISO Abbreviation: Toxicon Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Oxford ; New York : Pergamon Press, c1962- |
| MeSH Terms: | Metal Nanoparticles*; Gold/*chemistry; Elapid Venoms/analysis ; Viper Venoms/analysis ; Enzyme-Linked Immunosorbent Assay ; India ; Snake Bites |
| Abstract: | In this study, we have developed a gold nanoparticle based simple, rapid lateral flow assay (LFA) for detection of Indian Cobra venom (CV) and Russell's viper venom (RV). Presently, there is no rapid, reliable, and field diagnostic test available in India, where snake bite cases are rampant. Therefore, this test has an immense potential from the public health point of view. The test is based on the principle of the paper immunochromatography assay for detection of two snake venom species using polyvalent antisnake venom antibodies (ASVA) raised in equines and species-specific antibodies (SSAbs) against venoms raised in rabbits for conjugation and impregnation respectively. The developed, snake envenomation detection immunoassay (SEDIA) was rapid, selective, and sensitive to detect venom concentrations up to 0.1 ng/ml. The functionality of SEDIA strips was confirmed by experimental envenomation in mice and the results obtained were specific for the corresponding venom. The SEDIA has a potential to be a field diagnostic test to detect snake envenomation and assist in saving lives of snakebite victims.; (Copyright © 2016 Elsevier Ltd. All rights reserved.) |
| Comments: | Erratum in: Toxicon. 2017 Jan;125:121. doi: 10.1016/j.toxicon.2016.10.009.. (PMID: 27951506) |
| Contributed Indexing: | Keywords: Gold nanoparticles; Lateral flow assay; Rapid detection; SEDIA; Snake envenomation |
| Substance Nomenclature: | 0 (Elapid Venoms); 0 (Viper Venoms); 7440-57-5 (Gold) |
| Entry Date(s): | Date Created: 20160706 Date Completed: 20170622 Latest Revision: 20260127 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.toxicon.2016.06.023 |
| PMID: | 27377230 |
| Database: | MEDLINE |
Journal Article