Generation and characterization of tamoxifen-inducible Pax9-CreER knock-in mice using CrispR/Cas9.
| Title: | Generation and characterization of tamoxifen-inducible Pax9-CreER knock-in mice using CrispR/Cas9. |
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| Authors: | Feng J; Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA, 90033, USA.; Jing J; Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA, 90033, USA.; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.; Sanchez-Lara PA; Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA, 90033, USA.; Center for Personalized Medicine, Children's Hospital Los Angeles, Los Angeles, CA, 90027, USA.; Department of Pathology & Pediatrics, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90089, USA.; Bootwalla MS; Center for Personalized Medicine, Children's Hospital Los Angeles, Los Angeles, CA, 90027, USA.; Buckley J; Center for Personalized Medicine, Children's Hospital Los Angeles, Los Angeles, CA, 90027, USA.; Department of Preventive Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90089, USA.; Wu N; USC Norris Comprehensive Cancer Center Transgenic/Knockout Rodent Core Facility, Los Angeles, CA, 90089, USA.; Yan Y; USC Norris Comprehensive Cancer Center Transgenic/Knockout Rodent Core Facility, Los Angeles, CA, 90089, USA.; Chai Y; Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA, 90033, USA. ychai@usc.edu. |
| Source: | Genesis (New York, N.Y. : 2000) [Genesis] 2016 Sep; Vol. 54 (9), pp. 490-6. Date of Electronic Publication: 2016 Jul 26. |
| Publication Type: | Journal Article; Research Support, N.I.H., Extramural |
| Language: | English |
| Journal Info: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 100931242 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-968X (Electronic) Linking ISSN: 1526954X NLM ISO Abbreviation: Genesis Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New York, NY : Wiley-Liss, c2000- |
| MeSH Terms: | CRISPR-Cas Systems*; Gene Knock-In Techniques/*methods; Integrases/genetics ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; PAX9 Transcription Factor/genetics ; Tamoxifen/pharmacology ; Transcriptional Activation/drug effects ; Animals ; Mice ; Mice, Inbred C57BL ; Red Fluorescent Protein |
| Abstract: | Pax9 encodes a paired-box homeodomain (Pax) transcription factor and is critical for the development of multiple organs. Using CrispR/Cas9-mediated homologous directed repair (HDR), we generated a new Pax9-CreER knock-in mouse line in which the CreER(T2) fusion protein is produced after synthesis of endogenous Pax9 protein. We found that tdTomato reporter expression in Pax9-CreER;tdTomato reporter mice is detectable in a similar pattern to the endogenous Pax9 expression, faithfully recapitulating the Pax9 expression domains throughout the embryo and in the adult mouse. At early embryonic stages, the tdTomato reporter is expressed first in the pharyngeal pouch region and later in the craniofacial mesenchyme, somites, limbs, and lingual papillae in the adult tongue. These results demonstrate that this new Pax9-CreER knock-in mouse line can be used for lineage tracing and genetic targeting of Pax9-expressing cells and their progeny in a temporally and spatially controlled manner during development and organogenesis.; (© 2016 Wiley Periodicals, Inc.) |
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| Grant Information: | R01 DE025221 United States DE NIDCR NIH HHS; R37 DE012711 United States DE NIDCR NIH HHS; R90 DE022528 United States DE NIDCR NIH HHS; R01 DE012711 United States DE NIDCR NIH HHS; R01 DE022503 United States DE NIDCR NIH HHS |
| Contributed Indexing: | Keywords: Pax9; Pax9-CreER; genetic labeling; mouse development |
| Substance Nomenclature: | EC 2.7.7.- (Integrases); 0 (Luminescent Proteins); 0 (PAX9 Transcription Factor); 094ZI81Y45 (Tamoxifen); 0 (Red Fluorescent Protein); EC 2.7.7.- (Cre recombinase) |
| Entry Date(s): | Date Created: 20160707 Date Completed: 20170518 Latest Revision: 20260127 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC5021577 |
| DOI: | 10.1002/dvg.22956 |
| PMID: | 27381449 |
| Database: | MEDLINE |
Journal Article; Research Support, N.I.H., Extramural