Characterization of prophages containing "evolved" Dit/Tal modules in the genome of Lactobacillus casei BL23.
| Title: | Characterization of prophages containing "evolved" Dit/Tal modules in the genome of Lactobacillus casei BL23. |
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| Authors: | Dieterle ME; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina.; Fina Martin J; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina.; Durán R; Unidad de Bioquímica y Proteómica Analíticas, Institut Pasteur de Montevideo, Montevideo, Uruguay.; Nemirovsky SI; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina.; Sanchez Rivas C; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina.; Bowman C; Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, USA.; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA.; Russell D; Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, USA.; Hatfull GF; Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, USA.; Cambillau C; Architecture et Fonction des Macromolécules Biologiques, UMR 7257 Centre National de la Recherche Scientifique, Marseille, France.; Architecture et Fonction des Macromolécules Biologiques, UMR 7257 Aix-Marseille Université, Marseille, France.; Piuri M; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina. mpiuri@qb.fcen.uba.ar. |
| Source: | Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2016 Nov; Vol. 100 (21), pp. 9201-9215. Date of Electronic Publication: 2016 Jul 23. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Berlin ; New York : Springer International, c1984- |
| MeSH Terms: | Virus Activation*; Lacticaseibacillus casei/*genetics ; Lacticaseibacillus casei/*virology ; Prophages/*genetics ; Prophages/*physiology; Mitomycin/metabolism ; Nucleic Acid Synthesis Inhibitors/metabolism ; Viral Tail Proteins/genetics ; Food Microbiology |
| Abstract: | Lactic acid bacteria (LAB) have many applications in food and industrial fermentations. Prophage induction and generation of new virulent phages is a risk for the dairy industry. We identified three complete prophages (PLE1, PLE2, and PLE3) in the genome of the well-studied probiotic strain Lactobacillus casei BL23. All of them have mosaic architectures with homologous sequences to Streptococcus, Lactococcus, Lactobacillus, and Listeria phages or strains. Using a combination of quantitative real-time PCR, genomics, and proteomics, we showed that PLE2 and PLE3 can be induced-but with different kinetics-in the presence of mitomycin C, although PLE1 remains as a prophage. A structural analysis of the distal tail (Dit) and tail associated lysin (Tal) baseplate proteins of these prophages and other L. casei/paracasei phages and prophages provides evidence that carbohydrate-binding modules (CBM) located within these "evolved" proteins may replace receptor binding proteins (RBPs) present in other well-studied LAB phages. The detailed study of prophage induction in this prototype strain in combination with characterization of the proteins involved in host recognition will facilitate the design of new strategies for avoiding phage propagation in the dairy industry. |
| Contributed Indexing: | Keywords: Bacteriophage; Baseplate; Lactobacillus casei; Prophage |
| Substance Nomenclature: | 0 (Nucleic Acid Synthesis Inhibitors); 0 (Viral Tail Proteins); 50SG953SK6 (Mitomycin) |
| Entry Date(s): | Date Created: 20160725 Date Completed: 20170118 Latest Revision: 20221207 |
| Update Code: | 20260130 |
| DOI: | 10.1007/s00253-016-7727-x |
| PMID: | 27448399 |
| Database: | MEDLINE |
Journal Article