New Insights into the Enterococcus faecium and Streptococcus gallolyticus subsp. gallolyticus Host Interaction Mechanisms.
| Title: | New Insights into the Enterococcus faecium and Streptococcus gallolyticus subsp. gallolyticus Host Interaction Mechanisms. |
|---|---|
| Authors: | Sánchez-Díaz AM; Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.; Red Española de Investigación en Patología Infecciosa (REIPI), Sevilla, Spain.; Romero-Hernández B; Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.; Conde-Moreno E; Grupo de Biomarcadores y Dianas Terapéuticas, Hospital Universitario Ramón y Cajal and IRYCIS, Madrid, Spain.; Kwak YK; Microbiology Tumor and Cell Biology Department (MTC), Karolinska Institutet, Stockholm, Sweden.; Zamora J; Unidad de Bioestadística Clínica, Hospital Universitario Ramón y Cajal and IRYCIS, Madrid, Spain.; Colque-Navarro P; Microbiology Tumor and Cell Biology Department (MTC), Karolinska Institutet, Stockholm, Sweden.; Möllby R; Microbiology Tumor and Cell Biology Department (MTC), Karolinska Institutet, Stockholm, Sweden.; Ruiz-Garbajosa P; Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.; Red Española de Investigación en Patología Infecciosa (REIPI), Sevilla, Spain.; Cantón R; Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.; Red Española de Investigación en Patología Infecciosa (REIPI), Sevilla, Spain.; García-Bermejo L; Grupo de Biomarcadores y Dianas Terapéuticas, Hospital Universitario Ramón y Cajal and IRYCIS, Madrid, Spain.; Del Campo R; Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.; Red Española de Investigación en Patología Infecciosa (REIPI), Sevilla, Spain. |
| Source: | PloS one [PLoS One] 2016 Jul 27; Vol. 11 (7), pp. e0159159. Date of Electronic Publication: 2016 Jul 27 (Print Publication: 2016). |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: San Francisco, CA : Public Library of Science |
| MeSH Terms: | Host-Pathogen Interactions*; Enterococcus faecium/*physiology ; Streptococcus gallolyticus/*physiology; Biofilms ; Caco-2 Cells ; Humans |
| Abstract: | Enterococcus faecium and Streptococcus gallolyticus subsp. gallolyticus (S. gallolyticus) were classically clustered into the Lancefield Group D streptococci and despite their taxonomic reclassification still share a similar genetic content and environment. Both species are considered as opportunistic pathogens. E. faecium is often associated with nosocomial bacteraemia, and S. gallolyticus is sporadically found in endocarditis of colorectal cancer patients. In both cases, the source of infection is commonly endogenous with a translocation process that launches through the intestinal barrier. To get new insights into the pathological processes preceding infection development of both organisms, we used an in vitro model with Caco-2 cells to study and compare the adhesion, invasion and translocation inherent abilities of 6 E. faecium and 4 S. gallolyticus well-characterized isolates. Additionally, biofilm formation on polystyrene, collagen I and IV was also explored. Overall results showed that E. faecium translocated more efficiently than S. gallolyticus, inducing a destabilization of the intestinal monolayer. Isolates Efm106, Efm121 and Efm113 (p < .001 compared to Ef222) exhibited the higher translocation ability and were able to adhere 2-3 times higher than S. gallolyticus isolates. Both species preferred the collagen IV coated surfaces to form biofilm but the S. gallolyticus structures were more compact (p = .01). These results may support a relationship between biofilm formation and vegetation establishment in S. gallolyticus endocarditis, whereas the high translocation ability of E. faecium high-risk clones might partially explain the increasing number of bacteraemia. |
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| Entry Date(s): | Date Created: 20160728 Date Completed: 20170717 Latest Revision: 20181113 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC4963119 |
| DOI: | 10.1371/journal.pone.0159159 |
| PMID: | 27463203 |
| Database: | MEDLINE |
Journal Article