Effect of X-ray irradiation on ancient DNA in sub-fossil bones - Guidelines for safe X-ray imaging.
| Title: | Effect of X-ray irradiation on ancient DNA in sub-fossil bones - Guidelines for safe X-ray imaging. |
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| Authors: | Immel A; Department of Archaeogenetics, Max Planck Institute for the Science of Human History, Jena, Germany.; Institute for Archaeological Sciences, Archaeo- and Palaeogenetics, University of Tübingen, Tübingen, Germany.; Le Cabec A; Department of Human Evolution, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.; European Synchrotron Radiation Facility, Grenoble, France.; Bonazzi M; Institute of Clinical Molecular Biology, Kiel University, Kiel, Germany.; Herbig A; Department of Archaeogenetics, Max Planck Institute for the Science of Human History, Jena, Germany.; Temming H; Department of Human Evolution, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.; Schuenemann VJ; Institute for Archaeological Sciences, Archaeo- and Palaeogenetics, University of Tübingen, Tübingen, Germany.; Senckenberg Center for Human Evolution and Palaeoenvironment, University of Tübingen, Tübingen, Germany.; Bos KI; Department of Archaeogenetics, Max Planck Institute for the Science of Human History, Jena, Germany.; Langbein F; Institute for Archaeological Sciences, Archaeo- and Palaeogenetics, University of Tübingen, Tübingen, Germany.; Harvati K; Senckenberg Center for Human Evolution and Palaeoecology, Palaeoanthropology, University of Tübingen, Tübingen, Germany.; Bridault A; CNRS UMR 7041 ArScAn, Equipe Archéologies environnementales, F-92023 Nanterre Cedex, France.; Pion G; Association départementale pour la recherche archéologique en Savoie, F-73230 Saint-Alban-Leysse, France.; Julien MA; Centre for the Archaeology of Human Origins, Archaeology Department, University of Southampton, Southampton, UK.; Unité Histoire naturelle de l'Homme préhistorique (UMR 7194), Sorbonne Universités, Muséum national d'Histoire naturelle, CNRS, Paris, France.; Krotova O; Department of Stone Age, Institute of Archaeology, National Ukrainian Academy of Science, Kiev, Ukraine.; Conard NJ; Senckenberg Center for Human Evolution and Palaeoenvironment, University of Tübingen, Tübingen, Germany.; Münzel SC; Institute for Archaeological Sciences, Archaeozoology, University of Tübingen, Tübingen, Germany.; Drucker DG; Department of Geosciences, Palaeobiology, University of Tübingen, Tübingen, Germany.; Viola B; Department of Human Evolution, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.; Department of Anthropology, University of Toronto, Toronto, Canada.; Hublin JJ; Department of Human Evolution, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.; Tafforeau P; European Synchrotron Radiation Facility, Grenoble, France.; Krause J; Department of Archaeogenetics, Max Planck Institute for the Science of Human History, Jena, Germany.; Institute for Archaeological Sciences, Archaeo- and Palaeogenetics, University of Tübingen, Tübingen, Germany.; Senckenberg Center for Human Evolution and Palaeoenvironment, University of Tübingen, Tübingen, Germany. |
| Source: | Scientific reports [Sci Rep] 2016 Sep 12; Vol. 6, pp. 32969. Date of Electronic Publication: 2016 Sep 12. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Nature Publishing Group, copyright 2011- |
| MeSH Terms: | DNA, Ancient*; Bone and Bones/*diagnostic imaging ; Fossils/*diagnostic imaging; Animals ; Dose-Response Relationship, Radiation ; Synchrotrons ; X-Rays |
| Abstract: | Sub-fossilised remains may still contain highly degraded ancient DNA (aDNA) useful for palaeogenetic investigations. Whether X-ray computed [micro-] tomography ([μ]CT) imaging of these fossils may further damage aDNA remains debated. Although the effect of X-ray on DNA in living organisms is well documented, its impact on aDNA molecules is unexplored. Here we investigate the effects of synchrotron X-ray irradiation on aDNA from Pleistocene bones. A clear correlation appears between decreasing aDNA quantities and accumulating X-ray dose-levels above 2000 Gray (Gy). We further find that strong X-ray irradiation reduces the amount of nucleotide misincorporations at the aDNA molecule ends. No representative effect can be detected for doses below 200 Gy. Dosimetry shows that conventional μCT usually does not reach the risky dose level, while classical synchrotron imaging can degrade aDNA significantly. Optimised synchrotron protocols and simple rules introduced here are sufficient to ensure that fossils can be scanned without impairing future aDNA studies. |
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| Substance Nomenclature: | 0 (DNA, Ancient) |
| Entry Date(s): | Date Created: 20160913 Date Completed: 20180518 Latest Revision: 20181113 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC5018823 |
| DOI: | 10.1038/srep32969 |
| PMID: | 27615365 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't