Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats.
| Title: | Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats. |
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| Authors: | Chies AB; Laboratory of Pharmacology, Marília Medical School, SP, Brazil. Electronic address: agnaldochies@hotmail.com.; de Oliveira PB; Laboratory of Pharmacology, Marília Medical School, SP, Brazil.; Rossignoli PS; Department of Physiotherapy and Occupational Therapy, São Paulo State University (UNESP), Marília, SP, Brazil.; Baptista RFF; Faculty Esefap of Tupã, SP, Brazil; Faculty of Alta Paulista (FAP), Tupã, SP, Brazil.; de Lábio RW; Laboratory of Genetic, Marília Medical School, São Paulo, Brazil.; Payão SLM; Laboratory of Genetic, Marília Medical School, São Paulo, Brazil. |
| Source: | Peptides [Peptides] 2017 Feb; Vol. 88, pp. 67-73. Date of Electronic Publication: 2016 Dec 21. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Elsevier Science Inc Country of Publication: United States NLM ID: 8008690 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-5169 (Electronic) Linking ISSN: 01969781 NLM ISO Abbreviation: Peptides Subsets: MEDLINE |
| Imprint Name(s): | Publication: New York, NY : Elsevier Science Inc.; Original Publication: Fayetteville, N. Y., Ankho International. |
| MeSH Terms: | Angiotensin II/*metabolism ; Endothelin-1/*genetics ; Mesenteric Veins/*metabolism ; Receptor, Endothelin A/*genetics ; Receptor, Endothelin B/*genetics; Angiotensin II/genetics ; Endothelin-1/metabolism ; Femoral Vein/drug effects ; Femoral Vein/metabolism ; Imidazoles/administration & dosage ; Indomethacin/administration & dosage ; Mesenteric Veins/drug effects ; NG-Nitroarginine Methyl Ester/administration & dosage ; Nitric Oxide/metabolism ; Prostaglandins/administration & dosage ; Pyridines/administration & dosage ; RNA, Messenger/genetics ; Vasoconstriction/drug effects ; Animals ; Gene Expression Regulation ; Organ Culture Techniques ; Physical Conditioning, Animal ; Rats |
| Abstract: | Exercise-induced adaptations of the modulating mechanisms that influence the angiotensin (Ang II) responses assume different features depending on the venous bed. In femoral veins, exercise mobilizes vasodilator prostanoids to cooperate with NO in order to maintain reduced Ang II responses. On the other hand, exercise's influence on the Ang II responses in veins that drain blood from the mesenteric region has been poorly described. Therefore, the present study aimed to identify the effects of a single bout of exercise, as well as exercise training, on the Ang II responses in mesenteric veins. The present study also aimed to investigate the involvement of prostanoids, NO and ET-1 in eventual exercise-induced modifications in these veins. To this end, mesenteric veins taken from resting-sedentary, exercised-sedentary, resting-trained and exercised-trained animals were studied in organ baths. In addition, the mRNA expression of prepro-endothelin-1 (ppET-1), as well as that of the ETA and ETB receptors, were quantified by real-time PCR in these veins. The results show that, either in absence or in presence of L-NAME, the Ang II responses were not different between groups. In the presence of indomethacin, higher Ang II responses were observed in the resting-trained animals than in the resting-sedentary animals. This difference, however, disappeared when L-NAME, BQ-123 or BQ-788 were added during incubation. In addition, no differences in ppET-1, ETA or ETB mRNA expression were observed between groups. Furthermore, in the presence of PD123,319, the Ang II responses in the exercised-sedentary animals were higher than those in the resting-sedentary animals. In conclusion, exercise training mobilizes endothelin-1 (ET-1) to reinforce the Ang II-induced responses mainly through ETA activation. On the other hand, vasodilator prostanoids are mobilized to act in parallel with NO in order to counterbalance the Ang II responses that have been potentiated by ET-1 in these trained animals.; (Copyright © 2016 Elsevier Inc. All rights reserved.) |
| Contributed Indexing: | Keywords: Angiotensin II; Endothelin-1; Exercise; Nitric oxide; Prostanoids; Vein |
| Substance Nomenclature: | 0 (Endothelin-1); 0 (Imidazoles); 0 (Prostaglandins); 0 (Pyridines); 0 (RNA, Messenger); 0 (Receptor, Endothelin A); 0 (Receptor, Endothelin B); 0 (ednrb protein, rat); 11128-99-7 (Angiotensin II); 130663-39-7 (PD 123319); 31C4KY9ESH (Nitric Oxide); V55S2QJN2X (NG-Nitroarginine Methyl Ester); XXE1CET956 (Indomethacin) |
| Entry Date(s): | Date Created: 20161226 Date Completed: 20171129 Latest Revision: 20180718 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.peptides.2016.12.013 |
| PMID: | 28012855 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't