Cell survival and protein secretion associated with Golgi integrity in response to Golgi stress-inducing agents.
| Title: | Cell survival and protein secretion associated with Golgi integrity in response to Golgi stress-inducing agents. |
|---|---|
| Authors: | Ignashkova TI; Metabolism and Signaling in Cancer, BioMed X Innovation Center, Heidelberg, Germany.; Gendarme M; Metabolism and Signaling in Cancer, BioMed X Innovation Center, Heidelberg, Germany.; Peschk K; Medicinal Chemistry, Merck Biopharma, Merck KGaA, Darmstadt, Germany.; Eggenweiler HM; Medicinal Chemistry, Merck Biopharma, Merck KGaA, Darmstadt, Germany.; Lindemann RK; Translational Innovation Platform Oncology, Merck Biopharma, Merck KGaA, Darmstadt, Germany.; Reiling JH; Metabolism and Signaling in Cancer, BioMed X Innovation Center, Heidelberg, Germany. |
| Source: | Traffic (Copenhagen, Denmark) [Traffic] 2017 Aug; Vol. 18 (8), pp. 530-544. Date of Electronic Publication: 2017 Jun 30. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Wiley Country of Publication: England NLM ID: 100939340 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0854 (Electronic) Linking ISSN: 13989219 NLM ISO Abbreviation: Traffic Subsets: MEDLINE |
| Imprint Name(s): | Publication: Oxford : Wiley; Original Publication: Copenhagen, Denmark ; Malden, MA : Munksgaard, c2000- |
| MeSH Terms: | Cell Survival* ; Stress, Physiological*; Golgi Apparatus/*metabolism; ADP-Ribosylation Factors/drug effects ; ADP-Ribosylation Factors/metabolism ; Brefeldin A/pharmacology ; Naphthols/pharmacology ; Pyridines/pharmacology ; Quinolines/pharmacology ; Cell Line, Tumor ; HEK293 Cells ; Humans |
| Abstract: | The Golgi apparatus is part of the secretory pathway and of central importance for modification, transport and sorting of proteins and lipids. ADP-ribosylation factors, whose activation can be blocked by brefeldin A (BFA), play a major role in functioning of the Golgi network and regulation of membrane traffic and are also involved in proliferation and migration of cancer cells. Due to high cytotoxicity and poor bioavailability, BFA has not passed the preclinical stage of drug development. Recently, AMF-26 and golgicide A have been described as novel inhibitors of the Golgi system with antitumor or bactericidal properties. We provide here further evidence that AMF-26 closely mirrors the mode of action of BFA but is less potent. Using several human cancer cell lines, we studied the effects of AMF-26, BFA and golgicide A on cell homeostasis including Golgi structure, endoplasmic reticulum (ER) stress markers, secretion and viability, and found overall a significant correlation between these parameters. Furthermore, modulation of ADP-ribosylation factor expression has a profound impact on Golgi organization and survival in response to Golgi stress inducers.; (© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.) |
| Contributed Indexing: | Keywords: AMF-26; ARF GTPase; Golgi fragmentation; Golgi stress; brefeldin A; golgicide A |
| Substance Nomenclature: | 0 (N-(pyridine-3-ylmethyl)-5-(7-hydroxy-2,6,8-trimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalene-1-yl)-2-methylpenta-2,4-dienamide); 0 (Naphthols); 0 (Pyridines); 0 (Quinolines); 0 (golgicide A); 20350-15-6 (Brefeldin A); EC 3.6.5.2 (ADP-Ribosylation Factors) |
| Entry Date(s): | Date Created: 20170510 Date Completed: 20180402 Latest Revision: 20180410 |
| Update Code: | 20260130 |
| DOI: | 10.1111/tra.12493 |
| PMID: | 28485883 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't