CDK5 and MAPT Gene Expression in Alzheimer's Disease Brain Samples.
| Title: | CDK5 and MAPT Gene Expression in Alzheimer's Disease Brain Samples. |
|---|---|
| Authors: | Fukasawa JT; Marilia School of Medicine (Faculdade de Medicina de Marilia), Marilia Sao Paulo. Brazil.; de Labio RW; Marilia School of Medicine (Faculdade de Medicina de Marilia), Marilia Sao Paulo. Brazil.; Rasmussen LT; Sagrado Coracao University (Universidade Sagrado Coracao), Bauru, Sao Paulo. Brazil.; de Oliveira LC; Marilia School of Medicine (Faculdade de Medicina de Marilia), Marilia Sao Paulo. Brazil.; Chen E; Federal University of Sao Paulo (Universidade Federal de Sao Paulo), Sao Paulo. Brazil.; Villares J; Federal University of Sao Paulo (Universidade Federal de Sao Paulo), Sao Paulo. Brazil.; Tureck G; Bell Canada Brain Bank,Douglas Mental Health University Institute, Montreal Quebec. Canada.; de Arruda C Smith M; Federal University of Sao Paulo (Universidade Federal de Sao Paulo), Sao Paulo. Brazil.; Payao SLM; Marilia School of Medicine (Faculdade de Medicina de Marilia), Marilia Sao Paulo. Brazil. |
| Source: | Current Alzheimer research [Curr Alzheimer Res] 2018; Vol. 15 (2), pp. 182-186. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Bentham Science Publishers Country of Publication: United Arab Emirates NLM ID: 101208441 Publication Model: Print Cited Medium: Internet ISSN: 1875-5828 (Electronic) Linking ISSN: 15672050 NLM ISO Abbreviation: Curr Alzheimer Res Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Saif Zone, Sharjah, U.A.E. ; San Francisco, CA : Bentham Science Publishers, c2004- |
| MeSH Terms: | Alzheimer Disease/*metabolism ; Auditory Cortex/*metabolism ; Cyclin-Dependent Kinase 5/*metabolism ; Entorhinal Cortex/*metabolism ; Hippocampus/*metabolism ; tau Proteins/*metabolism; RNA, Messenger/metabolism ; Aged ; Aged, 80 and over ; Female ; Gene Expression ; Humans ; Male |
| Abstract: | Background: Alzheimer's disease (AD) is a neurodegenerative disorder characterized by extracellular amyloid plaque and neurofibrillary tangles in the brain. Studies have shown that neurons are able to re-enter the cell cycle, but not enough to enable full replication. This leads to cell death and consequent neurodegeneration.; Objective: This study aimed to characterize the expression of the MAPT gene and CDK5 (the gene involved in cell cycle regulation) in brain samples from patients with AD and controls.; Method: The real-time-PCR technique was used to characterize 150 samples from three areas of the brain (entorhinal cortex, auditory cortex, and hippocampus) of 26 AD patients and 24 healthy elderly subjects.; Results: When the brain samples were analyzed collectively, a decrease in CDK5 and MAPT gene expression was found in AD patients. When each groups' samples were separated by area of the brain and compared, significant differences were found in CDK5 expression in the hippocampus and the entorhinal cortex. In both cases, mRNA was lower in the AD group (p=0.0001); however, the same analysis using the MAPT gene revealed no significant statistical differences. No statistical differences were found when gene expression was compared between the different regions of the brain within each group.; Conclusion: These results may contribute to a better understanding of the involvement of CDK5 and MAPT genes in AD in that they consider different areas of the brain that are affected differently based on disease progression. The main challenge is to establish an effective therapy for this debilitating disease in the future.; (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.) |
| Contributed Indexing: | Keywords: Alzheimer's disease; CDK5; MAPT; brain; cell cycle; gene expression |
| Substance Nomenclature: | 0 (MAPT protein, human); 0 (RNA, Messenger); 0 (tau Proteins); EC 2.7.11.1 (Cyclin-Dependent Kinase 5); EC 2.7.11.22 (CDK5 protein, human) |
| Entry Date(s): | Date Created: 20170718 Date Completed: 20190304 Latest Revision: 20190304 |
| Update Code: | 20260130 |
| DOI: | 10.2174/1567205014666170713160407 |
| PMID: | 28714390 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't