Adaptation of Methanogenic Inocula to Anaerobic Digestion of Maize Silage.
| Title: | Adaptation of Methanogenic Inocula to Anaerobic Digestion of Maize Silage. |
|---|---|
| Authors: | Wojcieszak M; Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Warsaw, Poland.; Pyzik A; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.; Poszytek K; Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Warsaw, Poland.; Krawczyk PS; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.; Sobczak A; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.; Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland.; Lipinski L; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.; Roubinek O; Institute of Nuclear Chemistry and Technology, Warsaw, Poland.; Palige J; Institute of Nuclear Chemistry and Technology, Warsaw, Poland.; Sklodowska A; Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Warsaw, Poland.; Drewniak L; Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Warsaw, Poland. |
| Source: | Frontiers in microbiology [Front Microbiol] 2017 Sep 28; Vol. 8, pp. 1881. Date of Electronic Publication: 2017 Sep 28 (Print Publication: 2017). |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Lausanne : Frontiers Research Foundation |
| Abstract: | A well-balanced microbial consortium is crucial for efficient biogas production. In turn, one of a major factor that influence on the structure of anaerobic digestion (AD) consortium is a source of microorganisms which are used as an inoculum. This study evaluated the influence of inoculum sources (with various origin) on adaptation of a biogas community and the efficiency of the biomethanization of maize silage. As initial inocula for AD of maize silage the samples from: (i) an agricultural biogas plant (ABP) which utilizes maize silage as a main substrate, (ii) cattle slurry (CS), which contain elevated levels of lignocelluloses materials, and (iii) raw sewage sludge (RSS) with low content of plant origin materials were used. The adaptation of methanogenic consortia was monitored during a series of passages, and the functionality of the adapted consortia was verified through start-up operation of AD in two-stage reactors. During the first stages of the adaptation phase, methanogenic consortia occurred very slowly, and only after several passages did the microbial community adapts to allow production of biogas with high methane content. The ABP consortium revealed highest biogas production in the adaptation and in the start-up process. The biodiversity dynamics monitored during adaptation and start-up process showed that community profile changed in a similar direction in three studied consortia. Native communities were very distinct to each other, while at the end of the Phase II of the start-up process microbial diversity profile was similar in all consortia. All adopted bacterial communities were dominated by representatives of Porphyromonadaceae, Rikenellaceae, Ruminococcaceae, and Synergistaceae. A shift from low acetate-preferring acetoclastic Methanosaetaceae (ABP and RSS) and/or hydrogenotrophic Archaea, e.g., Methanomicrobiaceae (CS) prevailing in the inoculum samples to larger populations of high acetate-preferring acetoclastic Methanosarcinaceae was observed by the end of the experiment. As a result, three independent, functional communities that syntrophically produced methane from acetate (primarily) and H2/CO2, methanol and methylamines were adapted. This study provides new insights into the specific process by which different inocula sampled from typical methanogenic environments that are commonly used to initiate industrial installations gradually adapted to allow biogas production from maize silage. |
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| Contributed Indexing: | Keywords: anaerobic digestion; biodiversity dynamics; inoculum source; maize silage; methanogenic consortia |
| Entry Date(s): | Date Created: 20171017 Latest Revision: 20201001 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC5625012 |
| DOI: | 10.3389/fmicb.2017.01881 |
| PMID: | 29033919 |
| Database: | MEDLINE |
Journal Article