The FUT2 secretor variant p.Trp154Ter influences serum vitamin B12 concentration via holo-haptocorrin, but not holo-transcobalamin, and is associated with haptocorrin glycosylation.
| Title: | The FUT2 secretor variant p.Trp154Ter influences serum vitamin B12 concentration via holo-haptocorrin, but not holo-transcobalamin, and is associated with haptocorrin glycosylation. |
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| Authors: | Velkova A; Medical Genomics and Metabolic Genetics Branch, National Human Genome Research Institute, Bethesda, MD 20892, USA.; Diaz JEL; Medical Genomics and Metabolic Genetics Branch, National Human Genome Research Institute, Bethesda, MD 20892, USA.; Pangilinan F; Medical Genomics and Metabolic Genetics Branch, National Human Genome Research Institute, Bethesda, MD 20892, USA.; Molloy AM; Department of Clinical Medicine, School of Medicine, Trinity College Dublin, Dublin 2, Ireland.; Mills JL; Division of Intramural Population Health Research, Eunice Kennedy Shriver NICHD, Bethesda, MD 20852, USA.; Shane B; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA.; Sanchez E; Medical Genomics and Metabolic Genetics Branch, National Human Genome Research Institute, Bethesda, MD 20892, USA.; Cunningham C; St. James's Hospital, Dublin 8, Ireland.; McNulty H; Northern Ireland Centre for Food and Health, University of Ulster, Coleraine BT52 1SA, Northern Ireland.; Cropp CD; Computational and Statistical Genomics Branch, National Human Genome Research Institute, Bethesda, MD 21224, USA.; Bailey-Wilson JE; Computational and Statistical Genomics Branch, National Human Genome Research Institute, Bethesda, MD 21224, USA.; Wilson AF; Computational and Statistical Genomics Branch, National Human Genome Research Institute, Bethesda, MD 21224, USA.; Brody LC; Medical Genomics and Metabolic Genetics Branch, National Human Genome Research Institute, Bethesda, MD 20892, USA. |
| Source: | Human molecular genetics [Hum Mol Genet] 2017 Dec 15; Vol. 26 (24), pp. 4975-4988. |
| Publication Type: | Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: IRL Press at Oxford University Press Country of Publication: England NLM ID: 9208958 Publication Model: Print Cited Medium: Internet ISSN: 1460-2083 (Electronic) Linking ISSN: 09646906 NLM ISO Abbreviation: Hum Mol Genet Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Oxford, England ; New York : IRL Press at Oxford University Press, c1992- |
| MeSH Terms: | Fucosyltransferases/*genetics ; Fucosyltransferases/*metabolism ; Transcobalamins/*genetics; Genome-Wide Association Study/methods ; Hep G2 Cells/metabolism ; Polymorphism, Single Nucleotide/genetics ; Transcobalamins/metabolism ; Vitamin B 12/analysis ; Vitamin B 12/blood ; Vitamin B 12/metabolism ; Vitamin B 12 Deficiency/metabolism ; Adult ; Aged ; Biological Transport ; Female ; Genetic Variation ; Genotype ; Glycosylation ; Humans ; Ireland ; Male ; Middle Aged ; Galactoside 2-alpha-L-fucosyltransferase |
| Abstract: | Vitamin B12 deficiency is common in older individuals. Circulating vitamin B12 concentration can be used to diagnose deficiency, but this test has substantial false positive and false negative rates. We conducted genome-wide association studies (GWAS) in which we resolved total serum vitamin B12 into the fractions bound to transcobalamin and haptocorrin: two carrier proteins with very different biological properties. We replicated reported associations between total circulating vitamin B12 concentrations and a common null variant in FUT2. This allele determines the secretor phenotype in which blood group antigens are found in non-blood body fluids. Vitamin B12 bound to haptocorrin (holoHC) remained highly associated with FUT2 rs601338 (p.Trp154Ter). Transcobalamin bound vitamin B12 (holoTC) was not influenced by this variant. HoloTC is the bioactive the form of the vitamin and is taken up by all tissues. In contrast, holoHC is only taken up by the liver. Using holoHC from individuals with known FUT2 genotypes, we demonstrated that FUT2 rs601338 genotype influences the glycosylation of haptocorrin. We then developed an experimental model demonstrating that holoHC is transported into cultured hepatic cells (HepG2) via the asialoglycoprotein receptor (ASGR). Our data challenge current published hypotheses on the influence of genetic variation on this clinically important measure and are consistent with a model in which FUT2 rs601338 influences holoHC by altering haptocorrin glycosylation, whereas B12 bound to non-glycosylated transcobalamin (i.e. holoTC) is not affected. Our findings explain some of the observed disparity between use of total B12 or holoTC as first-line clinical tests of vitamin B12 status.; (Published by Oxford University Press 2017. This work is written by US Government employees and is in the public domain in the US.) |
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| Substance Nomenclature: | EC 2.4.1.- (Fucosyltransferases); 0 (Transcobalamins); P6YC3EG204 (Vitamin B 12); EC 2.4.1.69 (Galactoside 2-alpha-L-fucosyltransferase) |
| Entry Date(s): | Date Created: 20171018 Date Completed: 20180720 Latest Revision: 20260127 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC5886113 |
| DOI: | 10.1093/hmg/ddx369 |
| PMID: | 29040465 |
| Database: | MEDLINE |
Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't