Exposure of human neurons to silver nanoparticles induces similar pattern of ABC transporters gene expression as differentiation: Study on proliferating and post-mitotic LUHMES cells.
| Title: | Exposure of human neurons to silver nanoparticles induces similar pattern of ABC transporters gene expression as differentiation: Study on proliferating and post-mitotic LUHMES cells. |
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| Authors: | Zuberek M; University of Lodz, Faculty of Biology and Environmental Protection, Department of Molecular Biophysics, 141/143 Pomorska St., 90-236, Lodz, Poland. Electronic address: zuberekmariusz@gmail.com.; Stępkowski TM; Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195, Warsaw, Poland. Electronic address: t.stepkowski@ichtj.waw.pl.; Kruszewski M; Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195, Warsaw, Poland; Department of Molecular Biology and Translational Research, Institute of Rural Health, Jaczewskiego 2, 20-090, Lublin, Poland; Faculty of Medicine, University of Information Technology and Management in Rzeszów, ul. Sucharskiego 2, 35-225, Rzeszów, Poland. Electronic address: m.kruszewski@ichtj.waw.pl.; Grzelak A; University of Lodz, Faculty of Biology and Environmental Protection, Department of Molecular Biophysics, 141/143 Pomorska St., 90-236, Lodz, Poland. Electronic address: agnieszka.grzelak@biol.uni.lodz.pl. |
| Source: | Mechanisms of ageing and development [Mech Ageing Dev] 2018 Apr; Vol. 171, pp. 7-14. Date of Electronic Publication: 2018 Feb 24. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Elsevier Science Ireland Country of Publication: Ireland NLM ID: 0347227 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-6216 (Electronic) Linking ISSN: 00476374 NLM ISO Abbreviation: Mech Ageing Dev Subsets: MEDLINE |
| Imprint Name(s): | Publication: Limerick : Elsevier Science Ireland; Original Publication: Lausanne, Elsevier Sequoia. |
| MeSH Terms: | Silver*/chemistry ; Silver*/pharmacology; ATP-Binding Cassette Transporters/*biosynthesis ; Cell Differentiation/*drug effects ; Gene Expression Regulation/*drug effects ; Metal Nanoparticles/*chemistry ; Mitosis/*drug effects ; Neurons/*metabolism; ATP-Binding Cassette Transporters/genetics ; Blood-Brain Barrier/cytology ; Blood-Brain Barrier/metabolism ; Neurons/cytology ; Humans |
| Abstract: | The Lund human mesencephalic (LUHMES) cell line originated from mesencephalon of 8-week human foetus is a renowned in vitro model of human dopaminergic neurons. After differentiation the cells exhibit dopaminergic and neuronal characteristics of biochemically and morphologically mature dopamine-like neurons. In this study we analysed expression of 42 genes from ABC transporter superfamily in both proliferating cells and differentiated neurons after treatment with silver nanoparticles. ABC transporter superfamily is especially known due to the involvement in multidrug resistance phenomenon, but also involvement in transport through blood-brain barrier. Our results indicate that in neurons silver nanoparticles mainly attenuate transporters responsible for maintaining asymmetry of cellular membrane and homeostasis of lipids and cholesterol. Our results revealed also that proliferating foetal brain cells are by far more susceptible to silver nanoparticles than differentiated neurons.; (Copyright © 2018 Elsevier B.V. All rights reserved.) |
| Contributed Indexing: | Keywords: ABC transporter; Central nervous system; LUHMES cell line; Silver nanoparticles |
| Substance Nomenclature: | 0 (ATP-Binding Cassette Transporters); 3M4G523W1G (Silver) |
| Entry Date(s): | Date Created: 20180228 Date Completed: 20181211 Latest Revision: 20181211 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.mad.2018.02.004 |
| PMID: | 29486223 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't