Viability Test Device for anisakid nematodes.
| Title: | Viability Test Device for anisakid nematodes. |
|---|---|
| Authors: | Kroeger M; technet GmbH, Pestalozzistraße 8, D-70563 Stuttgart, Germany.; Karl H; Max Rubner-Institut, Palmaille 9, D-22767 Hamburg, Germany.; Simmler B; technet GmbH, Pestalozzistraße 8, D-70563 Stuttgart, Germany.; Singer P; technet GmbH, Pestalozzistraße 8, D-70563 Stuttgart, Germany. |
| Source: | Heliyon [Heliyon] 2018 Mar 06; Vol. 4 (3), pp. e00552. Date of Electronic Publication: 2018 Mar 06 (Print Publication: 2018). |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101672560 Publication Model: eCollection Cited Medium: Print ISSN: 2405-8440 (Print) Linking ISSN: 24058440 NLM ISO Abbreviation: Heliyon Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: London : Elsevier Ltd, [2015]- |
| Abstract: | Up to now the visual inspection of mobility of isolated anisakid larvae serves as a measure of viability and possible risk of infection. This paper presents a new method to rule out unreliability - caused by the temporary immobility of the larvae and by the human uncertainty factor of visual observation. By means of a Near infrared (NIR) imaging method, elastic curvature energies and geometric shape parameters were determined from contours, and used as a measure of viability. It was based on the modelling of larvae as a cylindrical membrane system. The interaction between curvatures, contraction of the longitudinal muscles, and inner pressure enabled the derivation of viability from stationary form data. From series of spectrally signed images within a narrow wavelength range, curvature data of the larvae were determined. Possible mobility of larvae was taken into account in statistical error variables. Experiments on individual living larvae, long-term observations of Anisakis larvae, and comparative studies of the staining method and the VTD measurements of larvae from the tissue of products confirmed the effectiveness of this method. The VTD differentiated clearly between live and dead nematode larvae isolated from marinated, deep-frozen and salted products. The VTD has been proven as excellent method to detect living anisakid nematode larvae in fishery products and is seen as useful tool for fish processing industry and control authorities. |
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| Contributed Indexing: | Keywords: Biophysics |
| Entry Date(s): | Date Created: 20180322 Latest Revision: 20201001 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC5857713 |
| DOI: | 10.1016/j.heliyon.2018.e00552 |
| PMID: | 29560464 |
| Database: | MEDLINE |
Journal Article