AIM/LIR-based fluorescent sensors-new tools to monitor mAtg8 functions.
| Title: | AIM/LIR-based fluorescent sensors-new tools to monitor mAtg8 functions. |
|---|---|
| Authors: | Zientara-Rytter K; a Section of Molecular Biology, Division of Biological Sciences , University of California San Diego , La Jolla , CA , USA.; Subramani S; a Section of Molecular Biology, Division of Biological Sciences , University of California San Diego , La Jolla , CA , USA. |
| Source: | Autophagy [Autophagy] 2018; Vol. 14 (6), pp. 1074-1078. Date of Electronic Publication: 2018 May 11. |
| Publication Type: | Journal Article; Research Support, N.I.H., Extramural |
| Language: | English |
| Journal Info: | Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101265188 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-8635 (Electronic) Linking ISSN: 15548627 NLM ISO Abbreviation: Autophagy Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2015- : Philadelphia, PA : Taylor & Francis; Original Publication: Georgetown, TX : Landes Bioscience, 2005- |
| MeSH Terms: | Autophagy-Related Protein 8 Family/*metabolism ; Fluorescent Dyes/*metabolism ; Microtubule-Associated Proteins/*metabolism; Mammals/metabolism ; Amino Acid Motifs ; Animals ; Fluorescence ; Humans ; Protein Binding |
| Abstract: | Macroautophagy/autophagy, a catabolic process by which cytoplasmic materials are degraded and recycled in lysosomes/vacuoles, remains a rapidly expanding research topic with the need for constantly improved methodologies to study each step of this pathway. Recently Lee and colleagues, as well as Stolz et al., independently reported the development of new AIM/LIR-based fluorescent sensors, which mark individual endogenous mammalian Atg8-family (mAtg8) proteins without affecting the autophagic flux. When expressed in cells, each sensor selectively recognizes individual mAtg8 isoforms and distinguishes mammalian MAP1LC3/LC3 proteins from the related GABARAPs. Such selectivity was achieved by using various LC3-interacting regions with high binding affinity to either a subgroup, or a specific, mAtg8 isoform as part of the sensor. Here we discuss the utility of these sensors in autophagy research and highlight their strengths, weaknesses and future directions. |
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| Grant Information: | R01 DK041737 United States DK NIDDK NIH HHS |
| Contributed Indexing: | Keywords: Atg8; Atg8-interacting motif; GABARAP; LC3; LC3-interacting region; autophagy assay; fluorescent probes; sensor |
| Substance Nomenclature: | 0 (Autophagy-Related Protein 8 Family); 0 (Fluorescent Dyes); 0 (Microtubule-Associated Proteins) |
| Entry Date(s): | Date Created: 20180512 Date Completed: 20191002 Latest Revision: 20191002 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC6103413 |
| DOI: | 10.1080/15548627.2018.1454238 |
| PMID: | 29749795 |
| Database: | MEDLINE |
Journal Article; Research Support, N.I.H., Extramural