HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion.
| Title: | HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion. |
|---|---|
| Authors: | MacDonald E; Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK.; Brown L; Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK.; Selvais A; Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK.; Liu H; Institute of Cancer Stem Cell, Dalian Medical University, Dalian, China.; Waring T; Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK.; Newman D; Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK.; Bithell J; Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK.; Grimes D; Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK.; Urbé S; Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK.; Clague MJ; Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK.; Zech T; Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK T.Zech@liverpool.ac.uk. |
| Source: | The Journal of cell biology [J Cell Biol] 2018 Jul 02; Vol. 217 (7), pp. 2549-2564. Date of Electronic Publication: 2018 Jun 11. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1540-8140 (Electronic) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New York : Rockefeller University Press |
| MeSH Terms: | Endosomal Sorting Complexes Required for Transport/*genetics ; Lysosomes/*genetics ; Matrix Metalloproteinase 14/*genetics ; Microfilament Proteins/*genetics ; Phosphoproteins/*genetics; Actins/genetics ; Cell Movement/genetics ; ErbB Receptors/genetics ; Lysosomes/metabolism ; Neoplasm Invasiveness/genetics ; Neoplasm Invasiveness/pathology ; Protein Transport/genetics ; Triple Negative Breast Neoplasms/genetics ; Triple Negative Breast Neoplasms/metabolism ; Triple Negative Breast Neoplasms/pathology ; Ubiquitination/genetics ; HeLa Cells ; Humans ; Protein Binding ; Proteolysis |
| Abstract: | Transmembrane proteins in the sorting endosome are either recycled to their point of origin or destined for lysosomal degradation. Lysosomal sorting is mediated by interaction of ubiquitylated transmembrane proteins with the endosomal sorting complex required for transport (ESCRT) machinery. In this study, we uncover an alternative role for the ESCRT-0 component hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) in promoting the constitutive recycling of transmembrane proteins. We find that endosomal localization of the actin nucleating factor Wiscott-Aldrich syndrome protein and SCAR homologue (WASH) requires HRS, which occupies adjacent endosomal subdomains. Depletion of HRS results in defective constitutive recycling of epidermal growth factor receptor and the matrix metalloproteinase MT1-MMP, leading to their accumulation in internal compartments. We show that direct interactions with endosomal actin are required for efficient recycling and use a model system of chimeric transferrin receptor trafficking to show that an actin-binding motif can counteract an ubiquitin signal for lysosomal sorting. Directed receptor recycling is used by cancer cells to achieve invasive migration. Accordingly, abrogating HRS- and actin-dependent MT1-MMP recycling results in defective matrix degradation and invasion of triple-negative breast cancer cells.; (© 2018 MacDonald et al.) |
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| Grant Information: | MR/M009114/1 United Kingdom MRC_ Medical Research Council; 105353 United Kingdom WT_ Wellcome Trust; BB/M011186/1/1797330 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; 105350/Z/14/A United Kingdom WT_ Wellcome Trust |
| Substance Nomenclature: | 0 (Actins); 0 (Endosomal Sorting Complexes Required for Transport); 0 (Microfilament Proteins); 0 (Phosphoproteins); 0 (WASH protein, human); 0 (hepatocyte growth factor-regulated tyrosine kinase substrate); EC 2.7.10.1 (EGFR protein, human); EC 2.7.10.1 (ErbB Receptors); EC 3.4.24.80 (MMP14 protein, human); EC 3.4.24.80 (Matrix Metalloproteinase 14) |
| Entry Date(s): | Date Created: 20180613 Date Completed: 20190520 Latest Revision: 20250530 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC6028553 |
| DOI: | 10.1083/jcb.201710051 |
| PMID: | 29891722 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't