A New Transport Solution for Parathyroid Allotransplantation: Effects on Cell Viability and Calcium-Sensing Receptors.
| Title: | A New Transport Solution for Parathyroid Allotransplantation: Effects on Cell Viability and Calcium-Sensing Receptors. |
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| Authors: | Goncu B; 1 Experimental Research Center, Bezmialem Vakif University , Istanbul, Turkey .; Yucesan E; 2 Institute of Life Sciences and Biotechnology, Bezmialem Vakif University , Istanbul, Turkey .; Ozdemir B; 1 Experimental Research Center, Bezmialem Vakif University , Istanbul, Turkey .; Basoglu H; 3 Department of Biophysics, Faculty of Medicine, Bezmialem Vakif University , Istanbul, Turkey .; Kandas NO; 4 Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Bezmialem Vakif University , Istanbul, Turkey .; Akbas F; 5 Department of Medical Biology, Faculty of Medicine, Bezmialem Vakif University , Istanbul, Turkey .; Aysan E; 6 Department of General Surgery, Faculty of Medicine, Bezmialem Vakif University , Istanbul, Turkey . |
| Source: | Biopreservation and biobanking [Biopreserv Biobank] 2018 Aug; Vol. 16 (4), pp. 278-284. Date of Electronic Publication: 2018 Jul 02. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Mary Ann Liebert Country of Publication: United States NLM ID: 101507284 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1947-5543 (Electronic) Linking ISSN: 19475543 NLM ISO Abbreviation: Biopreserv Biobank Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New Rochelle, NY : Mary Ann Liebert |
| MeSH Terms: | Cryopreservation/*methods ; Receptors, Calcium-Sensing/*metabolism; Amino Acids/chemistry ; Cell Survival/physiology ; Parathyroid Glands/cytology ; Parathyroid Hormone/chemistry ; Blotting, Western ; Cells, Cultured ; Humans ; Hydrogen-Ion Concentration |
| Abstract: | Background: Cold ischemia protects organs and tissues by slowing their metabolism, but it also causes ischemic injury. Minimizing cold ischemia has been an important goal in parathyroid auto- and allotransplantation, as well as the transplantation of other major organs. Parathyroid glands are responsible for calcium homeostasis by releasing parathormone (PTH) into the blood circulation. Functionality of a new parathyroid transport solution (NPTS) and effects on cell viability, PTH secretion, and calcium-sensing receptor (CaSR) levels during cold ischemia were evaluated.; Materials and Methods: A NPTS was prepared, and the pH was adjusted to a range of 7.2-7.4 and kept at +4°C until use. Seven patients with parathyroid hyperplasia secondary to chronic renal failure who were scheduled to undergo subtotal parathyroidectomy were enrolled in the study. Glands were cold-preserved in NPTS with different time intervals (0, 6, 12, 18, and 24 hours), and then parathyroid cell viability before and after cryopreservation, PTH secretion, and CaSR levels were determined.; Results: The mean cell viability before cryopreservation was 92.7% (range 89.2%-97.2%). There were no significant differences in cell viability rates before and after cryopreservation (p = 0.1168 and p = 0.4085, respectively), and CaSR levels (p = 0.5446) were not significant.; Conclusions: NPTS is a solution designed specifically for parathyroid tissue transplantation. This patent pending product can support cellular viability and PTH release, as well as protect CaSR functionality for up to 24 hours of cold ischemia. |
| Contributed Indexing: | Keywords: CaSR; PTH; cold ischemia; parathyroid tissue; preservation |
| Substance Nomenclature: | 0 (Amino Acids); 0 (Parathyroid Hormone); 0 (Receptors, Calcium-Sensing) |
| Entry Date(s): | Date Created: 20180703 Date Completed: 20181026 Latest Revision: 20181026 |
| Update Code: | 20260130 |
| DOI: | 10.1089/bio.2018.0008 |
| PMID: | 29963899 |
| Database: | MEDLINE |
Journal Article