LDL dinitrosyl iron complex acts as an iron donor in mouse macrophages.
| Title: | LDL dinitrosyl iron complex acts as an iron donor in mouse macrophages. |
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| Authors: | Lewandowska H; Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland. Electronic address: h.lewandowska@ichtj.waw.pl.; Stępkowski TM; Laboratory of Mitochondrial Biogenesis, Centre of New Technologies UW, Banacha 2c, 02-097 Warsaw, Poland.; Męczyńska-Wielgosz S; Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland.; Sikorska K; Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland.; Sadło J; Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland.; Dudek J; Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland.; Kruszewski M; Institute of Nuclear Chemistry and Technology, 16 Dorodna Str., 03-195 Warsaw, Poland; Faculty of Medicine, University of Information Technology and Management in Rzeszów, ul. Sucharskiego 2, 35-225 Rzeszów, Poland; Department of Molecular Biology and Translational Research, Institute of Rural Health, Jaczewskiego 2, 20-090 Lublin, Poland. |
| Source: | Journal of inorganic biochemistry [J Inorg Biochem] 2018 Nov; Vol. 188, pp. 29-37. Date of Electronic Publication: 2018 Aug 04. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: United States NLM ID: 7905788 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3344 (Electronic) Linking ISSN: 01620134 NLM ISO Abbreviation: J Inorg Biochem Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New York, Elsevier. |
| MeSH Terms: | Iron*/chemistry ; Iron*/pharmacology ; Lipoproteins, LDL*/chemistry ; Lipoproteins, LDL*/pharmacology ; Nitrogen Oxides*/chemistry ; Nitrogen Oxides*/pharmacology; Macrophages/*metabolism; Apolipoprotein B-100/chemistry ; Apolipoprotein B-100/pharmacology ; Macrophages/cytology ; Animals ; Mice ; RAW 264.7 Cells |
| Abstract: | [Fe(NO)2] - modified nanoparticles of low-density protein (DNICLDL) can serve as conveyors of iron in the form of stable complexes with ApoB100 protein. As reported recently, in human hepatoma cells DNICLDL significantly increased the total iron content, while showing low toxicity. In the present work, we focused on the effects of internalization of DNIC-modified lipoproteins in macrophages, with special regards to cytotoxicity. DNICLDL was administered to a model macrophage cell line, RAW 264.7. Administration of DNICLDL considerably increased total iron content. High increase of iron was accompanied by moderate toxicity. As shown by in vitro plasmid nicking assay, chelation of iron in the form of DNIC strongly reduced the iron-related reactive oxygen species (ROS) -induced DNA damage. In addition, DNICLDL, plausibly due to its NO-donating activity, did not induce inducible nitric oxide synthase (iNOS) expression, as opposed to other forms of low-density protein (LDL).; (Copyright © 2018 Elsevier Inc. All rights reserved.) |
| Substance Nomenclature: | 0 (Apolipoprotein B-100); 0 (Lipoproteins, LDL); 0 (Nitrogen Oxides); 68586-27-6 (dinitrosyl iron complex); E1UOL152H7 (Iron) |
| Entry Date(s): | Date Created: 20180818 Date Completed: 20190516 Latest Revision: 20190516 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.jinorgbio.2018.08.004 |
| PMID: | 30119015 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't