Comparative study of the effects of osteoprotegerin and testosterone on bone quality in male orchidectomised rats.
| Title: | Comparative study of the effects of osteoprotegerin and testosterone on bone quality in male orchidectomised rats. |
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| Authors: | Martín-Fernández M; Department of Biochemical Research, Institute of Sanitary Research Fundación Jiménez Díaz, Madrid, Spain.; Garzón-Márquez FM; Spinal Pathology Unit, Institute of Sanitary Research Fundación Jiménez Díaz, Spinal Pathology Unit, Madrid, Spain.; Díaz-Curiel M; Department of Internal Medicine, Institute of Sanitary Research Fundación Jiménez Díaz, Madrid, Spain.; Prieto-Potin I; Institute of Sanitary Research Fundación Jiménez Díaz, Pathological Anatomy, Madrid, Spain.; Alvarez-Galovich L; Spinal Pathology Unit, Institute of Sanitary Research Fundación Jiménez Díaz, Spinal Pathology Unit, Madrid, Spain.; Guede D; Technology based Firm Technological Park of Galicia, Ourense, Spain.; Caeiro-Rey JR; Department of Traumatology and Orthopedic Surgery, University Hospital of Santiago de Compostela, Santiago de Compostela, Spain.; De la Piedra C; Department of Biochemical Research, Institute of Sanitary Research Fundación Jiménez Díaz, Madrid, Spain. |
| Source: | The aging male : the official journal of the International Society for the Study of the Aging Male [Aging Male] 2020 Sep; Vol. 23 (3), pp. 189-201. Date of Electronic Publication: 2018 Aug 25. |
| Publication Type: | Comparative Study; Journal Article |
| Language: | English |
| Journal Info: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9808210 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1473-0790 (Electronic) Linking ISSN: 13685538 NLM ISO Abbreviation: Aging Male Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2015- : Abingdon, Oxford : Taylor & Francis; Original Publication: New York : Parthenon Pub., c1998- |
| MeSH Terms: | Bone Density*; Osteoprotegerin/*pharmacology ; Testosterone/*pharmacology; Femur/diagnostic imaging ; Femur/drug effects ; Femur/pathology ; Osteoprotegerin/administration & dosage ; Osteoprotegerin/blood ; Spine/diagnostic imaging ; Spine/drug effects ; Spine/pathology ; Testosterone/administration & dosage ; Testosterone/blood ; Animals ; Humans ; Male ; Orchiectomy ; Random Allocation ; Rats ; Rats, Wistar |
| Abstract: | Aim: The aim of this work is to compare the effects of osteoprotegerin (OPG) and testosterone on bone quality in a model of orchidectomised (ORX) rats.Methods: Three-month-old ORX or SHAM operated groups (n = 15 each group) were used. The SHAM and ORX groups received saline. There were two ORX groups, receiving OPG-Fc (10 mg/kg twice weekly) (ORX + OPG-Fc) or testosterone cypionate (1.7 mg/kg/weekly) for 8 weeks. After sacrifice, bone analysis by femoral and lumbar dual-energy X-ray absorptiometry and micro-computed tomography in femora were performed. Histological sections of vertebrae were dyed with hematoxylin-eosin or safranin. Serum osteocalcin (BGP), total alkaline phosphatase (ALP), and C-terminal telopeptide of type I collagen (CTX) were analyzed.Results: ORX resulted in femoral and vertebral bone loss and in microarchitectural deterioration. Treatment with OPG-Fc and testosterone recovered lumbar (L) and femoral (F) bone mineral densitometry bone mineral density (BMD) to SHAM levels. Femoral BMD was significantly higher after treatment with OPG-Fc than after testosterone treatment due to the presence of osteopetrotic changes in the metaphyseal region of long bones. Serum levels of ALP and CTX increased, while OPG levels were unchanged in ORX rats. Treatment with OPG-Fc decreased the levels of BGP, ALP, and CTX. Treatment with testosterone maintained biochemical markers of bone turnover at levels similar to or higher than those of ORX rats. |
| Contributed Indexing: | Keywords: Bone quality; OPG–Fc; orchidectomy; testosterone |
| Substance Nomenclature: | 0 (Osteoprotegerin); 3XMK78S47O (Testosterone) |
| Entry Date(s): | Date Created: 20180828 Date Completed: 20210816 Latest Revision: 20210816 |
| Update Code: | 20260130 |
| DOI: | 10.1080/13685538.2018.1499082 |
| PMID: | 30146916 |
| Database: | MEDLINE |
Comparative Study; Journal Article