Effect of red propolis extract isolated or encapsulated in nanoparticles on the in vitro culture of sheep preantral follicle: Impacts on antrum formation, mitochondrial activity and glutathione levels.
| Title: | Effect of red propolis extract isolated or encapsulated in nanoparticles on the in vitro culture of sheep preantral follicle: Impacts on antrum formation, mitochondrial activity and glutathione levels. |
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| Authors: | Nascimento TS; Tiradentes University, Technology and Research Institute, Aracaju-SE, Brazil.; Silva ISM; Tiradentes University, Technology and Research Institute, Aracaju-SE, Brazil.; Alves MCMA; Tiradentes University, Technology and Research Institute, Aracaju-SE, Brazil.; Gouveia BB; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina-PE, Brazil.; Barbosa LMR; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina-PE, Brazil.; Macedo TJS; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina-PE, Brazil.; Santos JMS; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina-PE, Brazil.; Monte APO; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina-PE, Brazil.; Matos MHT; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina-PE, Brazil.; Padilha FF; Tiradentes University, Technology and Research Institute, Aracaju-SE, Brazil.; Lima-Verde IB; Tiradentes University, Technology and Research Institute, Aracaju-SE, Brazil. |
| Source: | Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2019 Jan; Vol. 54 (1), pp. 31-38. Date of Electronic Publication: 2018 Nov 02. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Paul Parey Scientific Publishers Country of Publication: Germany NLM ID: 9015668 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1439-0531 (Electronic) Linking ISSN: 09366768 NLM ISO Abbreviation: Reprod Domest Anim Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Berlin ; Hamburg : Paul Parey Scientific Publishers, c1990- |
| MeSH Terms: | Cell Culture Techniques/*veterinary ; Culture Media/*chemistry ; Nanoparticles/*administration & dosage ; Ovarian Follicle/*drug effects ; Propolis/*pharmacology; Glutathione/analysis ; Mitochondria/physiology ; Ovarian Follicle/physiology ; Propolis/administration & dosage ; Propolis/chemistry ; Animals ; Female ; Oxidative Stress ; Sheep, Domestic |
| Abstract: | This study aimed to characterize the hydroethanolic extract of red propolis (HERP) and nanoparticles containing HERP for using as an additive in the culture medium of isolated ovine preantral follicles. HERP was characterized by high-performance liquid chromatography (HPLC) and determination of flavonoid content, and the nanoparticles by the mean particle diameter, polydispersity index (PI) and encapsulation efficiency (EE). The effect of HERP (10 and 20 ηg/ml-HERP10 and HERP20 groups) and nanoparticles (NP10 and NP20 groups) on isolated secondary follicles cultured in vitro for 12 days was observed by morphological evaluation, oxidative stress markers (reactive oxygen species-ROS and glutathione-GSH) and active mitochondria. HPLC showed formononetin as the major compound in the HERP (63.92 ± 0.21 μg/mL). The content of flavonoids ranged from 2.14% to 11.00%. The nanoparticles showed mean diameter of 287.5 ± 3.9 and 479 ± 18.1 ηm; PI of 0.117 ± 0.018 and 0.316 ± 0.039; and EE of 67.15% and 41%, respectively, for the NP10 and NP20 groups. After 12 days of culture, HERP20 and NP20 increased (p |
| Grant Information: | Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil); National Council for Scientific and Technological Development (CNPq, Brazil); Foundation for Supporting Research and Technological Innovation (FAPITEC, Sergipe, Brazil) |
| Contributed Indexing: | Keywords: antioxidants; development; nanotechnology; oocyte; ovine |
| Substance Nomenclature: | 0 (Culture Media); 9009-62-5 (Propolis); GAN16C9B8O (Glutathione) |
| Entry Date(s): | Date Created: 20180925 Date Completed: 20190122 Latest Revision: 20190122 |
| Update Code: | 20260130 |
| DOI: | 10.1111/rda.13347 |
| PMID: | 30246482 |
| Database: | MEDLINE |
Journal Article