Regulatory associations between the metabolism of sulfur-containing amino acids and xanthan biosynthesis in Xanthomonas campestris pv. campestris B100.
| Title: | Regulatory associations between the metabolism of sulfur-containing amino acids and xanthan biosynthesis in Xanthomonas campestris pv. campestris B100. |
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| Authors: | Schulte F; Department of Proteome and Metabolome Research - Bio27, Faculty of Biology, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany.; Leßmeier L; Chair of Genetics of Prokaryotes, Faculty of Biology, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany.; Voss J; Department of Proteome and Metabolome Research - Bio27, Faculty of Biology, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany.; Ortseifen V; Department of Proteome and Metabolome Research - Bio27, Faculty of Biology, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany.; Vorhölter FJ; Department of Proteome and Metabolome Research - Bio27, Faculty of Biology, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany.; Niehaus K; Department of Proteome and Metabolome Research - Bio27, Faculty of Biology, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany. |
| Source: | FEMS microbiology letters [FEMS Microbiol Lett] 2019 Jan 01; Vol. 366 (2). |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Oxford University Press Country of Publication: England NLM ID: 7705721 Publication Model: Print Cited Medium: Internet ISSN: 1574-6968 (Electronic) Linking ISSN: 03781097 NLM ISO Abbreviation: FEMS Microbiol Lett Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2015- : Oxford Oxford University Press; Original Publication: Amsterdam, Published by Elsevier/North Holland on behalf of the Federation of European Microbiological Societies. |
| MeSH Terms: | Gene Expression Regulation, Bacterial*; Amino Acids/*metabolism ; Polysaccharides, Bacterial/*biosynthesis ; Sulfur/*metabolism ; Xanthomonas campestris/*metabolism; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Xanthomonas campestris/genetics ; Xanthomonas campestris/growth & development |
| Abstract: | The γ-proteobacterium Xanthomonas campestris pv. campestris (Xcc) B100 synthesizes the exopolysaccharide xanthan, a commercially relevant thickening agent produced commonly by industrial scale fermentation. This work was inspired by the observation that methionine is an inhibitor of xanthan formation in growth experiments. Therefore, the global effects of methionine supplementation were characterized through cultivation experiments, genome-wide microarray hybridizations and qRT-PCR. Specific pull down of DNA-binding proteins by using the intergenic regions upstream of xanA, gumB and gumD led to the identification of six transcriptional regulators, among them the LysR-family transcriptional regulator CysB. An insertion mutant of this gene was analyzed by growth experiments, microarray experiments and qRT-PCR. Based on our experimental data, we developed a model that describes the methionine-dependent co-regulation of xanthan and sulfur-containing compounds in Xanthomonas. These data substantially contribute to better understand the impact of methionine as a compound in xanthan production media used in industrial fermentations. |
| Substance Nomenclature: | 0 (Amino Acids); 0 (Bacterial Proteins); 0 (Polysaccharides, Bacterial); 70FD1KFU70 (Sulfur); TTV12P4NEE (xanthan gum) |
| Entry Date(s): | Date Created: 20190117 Date Completed: 20190930 Latest Revision: 20190930 |
| Update Code: | 20260130 |
| DOI: | 10.1093/femsle/fnz005 |
| PMID: | 30649298 |
| Database: | MEDLINE |
Journal Article